Changes in the Chemical Compound and Sensory Profiles of Liberica Fermentation (Coffea liberica) with Cellulolytic Bacteria Alcaligenes sp and Exiguobacterium indicum
DOI:
https://doi.org/10.25186/.v19i.2258Abstract
Jambi Province, particularly in Tanjung Jabung Barat Regency, Indonesia, is a crucial producer of Liberica coffee known as Liberika Tungkal Komposit (Libtukom). This variety of Liberica coffee (Coffea liberica) thrives in lowland regions, particularly peatlands. It grows best in slightly acidic soil with a pH between 6.0 and 6.5, which helps the plant absorb nutrients and resist pests more effectively. A significant post-harvest method to improve the quality and yield of Liberica coffee involves fermentation using cellulolytic bacteria, Alcaligenes sp. And Exiguobacterium indicum, found in civet digestive tracts. Fermentation enhances the coffe’s flavor, aroma, and quality, mimicking that of civet coffee. The primary objective of this study was to refine the sensory characteristics of Liberica coffee, improve its proximate composition, preserve its bioactive compounds, and boost its antioxidant properties. The fermentation was conducted over 24 hours and 48 hours periods, with roasting temperatures set at light (170 ºC), medium (190 ºC), and dark (210 ºC). The process began with the preparation of bacterial inoculum, followed by fermentation, roasting, and grinding. Sensory evaluation, proximate analysis, and chemical profiling (via FTIR, LC-MS, antioxidant activity, and total phenolic content) were then performed. The findings revealed that fermented Liberica coffee exhibited improved sensory attributes, achieving specialty-grade status at medium roasting temperatures. There was a decrease in moisture and ash content, an increase in fat content, and a reduction in carbohydrate and protein levels. The FTIR analysis showed the preservation of existing compounds, while LC-MS identified bioactive components based on their retention times and molecular weights. Additionally, the fermented coffee demonstrated increased antioxidant activity and total phenolic content compared to non-fermented samples. This study highlights the potential of fermentation
technology to enhance the quality and health benefits of Liberica coffee significantly.
Key words: Coffee liberica; chemical compound; fermentation; sensory profile.
References
ANANDA, H. D. et al. Microencapsulation of fermented red palm oil with l. casei as nutracetical source. Jurnal Rekayasa Kimia & Lingkungan, 17(2):138-151, 2022.
ANHOFIAH, N.; RASYID, M. I. Quality characteristics of arabica longberry coffee at kbq baburrayyan resulting from semi-wash processing with the influence of long fermentation and long roasting. Jurnal Pertanian Agros, 25(1):364-375, 2023.
BALCÁZAR-ZUMAETA, C. R. et al. Metabolomics during the spontaneous fermentation in cocoa (Theobroma cacao L.): An exploraty review. Food Research International, 163:112190, 2023.
BERGHUIS, N. T.; MAULANA, P. Perbandingan metode ekstraksi asam lemak pada ampas kopi menggunakan metode soxhlet dan maserasi. Jurnal Kimia, 17(1):40, 2023.
BUDRYN, G. et al. Influence of roasting conditions on fatty acids and oxidative changes of Robusta coffee oil. European Journal of Lipid Science and Technology, 114(9):1052-1061, 2012.
DIVIŠ, P.; POŘÍZKA, J.; KŘÍKALA, J. The effect of coffee beans roasting on its chemical composition. Potravinarstvo Slovak Journal of Food Sciences, 13(1):344-350, 2019.
DUAJA, M. D.; SIMATUPANG, J.; KARTIKA, E. Strengthening group: Entrepreneurship reorientation toward development of liberica coffee. IOP Conference Series: Earth and Environmental Science, 391:012060, 2019.
FARAH, A.; DONANGELO, C. M. Phenolic compounds in coffee. Brazilian Journal of Plant Physiology, 18(1):23-36, 2006.
FEBRIANTO, N. A.; ZHU, F. Diversity in composition of bioactive compounds among 26 cocoa genotypes. Journal of Agricultural and Food Chemistry, 67(34):9501-9509, 2019.
HAILE, M.; KANG, W. H. Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation, 5(1):29, 2019.
HALUPI. Libtukom: Varietas kopi liberika anjuran untuk lahan gambut. Warta Pusat Penelitian Kopi dan Kakao di Indonesia, 26(1):1-6, 2016.
HU, G. et al. Effect of roasting degree of coffee beans on sensory evaluation: Research from the perspective of major chemical ingredients. Food Chemistry, 331:127329, 2020.
ISNIDAYU, A. V.; SUKARTIKO, A. C.; AINURI, M. Indicator of sensory attributes of speciality coffee originated from west java based on biochemical component. Jurnal Tanaman Industri dan Penyegar, 7(1):1, 2020.
ISWANTO, T. et al. Isolation and identification of caffeine-degrading bacteria from soil, coffee pulp waste and excreted coffee bean in Luwak feces. Biodiversitas, 20(6):1580-1587, 2019.
JOKANOVIĆ, M. R. et al. Changes of physical properties of coffee beans during roasting. Acta Periodica Technologica, 43:21-31, 2012.
KĘDZIERSKA-MATYSEK, M. et al. Relationships between the content of phenolic compounds and the antioxidant activity of polish honey varieties as a tool for botanical discrimination. Molecules, 26(6):1810, 2021.
KITZBERGER, C. S. G. et al. Flavor precursors and sensory attributes of coffee submitted to different post-harvest processing. AIMS Agriculture and Food, 5(4):700-714, 2020.
LATIEF, M. et al. Coffee improvement by wet fermentation using lactobacillus plantarum: sensory studies, proximate analysis, antioxidants, and chemical compounds. Jurnal Rekayasa Kimia & Lingkungan, 18(2):134-148, 2023.
LEE, L. W. et al. Coffee fermentation and flavor - An intricate and delicate relationship. Food Chemistry, 185:182-191, 2015.
MALIGAN, J. M.; ANGGONO, N. Z. Effect of fermentation time and roasting temperature on the sensory, chemical, and physical characterictics of wine coffee robusta tirtoyudo. Jurnal Pangan dan Agroindustri, 11(4):219-229, 2023.
MARTINEZ, S. J. et al. Effect of bacterial and yeast starters on the formation of volatile and organic acid compounds in coffee beans and selection of flavors markers precursors during wet fermentation. Frontiers in Microbiology, 10:1287, 2019.
MUBARAK, A. et al. Comparison of liberica and arabica coffee: Chlorogenic acid, caffeine, total phenolic and DPPH radical scavenging activity. Asian Journal of Agriculture and Biology, 7(1):130-136, 2019.
MULYARA, B. et al. Sensory properties and volatile compound profile of anaerobic fermented gayo arabica coffee in non-washed processing. Pelita Perkebunan, 37(3):239-254, 2021.
MUZAIFA, M. et al. Exploration study of indigenous civet (Paradoxorus hermaphroditus) bacteria: Isolates characterization and molecular identification. Agrointek: Jurnal Teknologi Industri Pertanian, 17(1):78-85, 2023.
NIZORI, A. et al. Influence of fermentation conditions on the antioxidant and physico-chemical of arabica coffee from Kerinci Region of Indonesia. Indonesian Food Science & Technology Journal, 5(1):34-38, 2021.
NUGROHO, D. Budidaya Kopi Liberika (Coffea liberica var Liberica) di Kabupaten Tanjung Jabung Barat, Jambi. Warta Pusat Penelitian Kopi dan Kakao di Indonesia, 27(1):9-14, 2015.
PAMUNGKAS, M. T.; MASRUKAN, M.; SAR, K. Pengaruh Suhu dan Lama Penyangraian (Roasting) terhadap sifat fisik dan kimia pada seduhan kopi arabika (Coffea arabica L) dari Kabupaten Gayo, Provinsi Aceh. Agrotech: Jurnal Ilmiah Teknologi Pertanian, 3(2):1-10, 2021.
PEREIRA, G. V. de M. et al. Microbial ecology and starter culture technology in coffee processing. Critical Reviews in Food Science and Nutrition, 57(13):2775-2788, 2017.
PRASTIWI, E. K. et al. The effect of fermentation time on the quality of mocaf (modified cassava flour) with raw material bokor genotype cassava. Journal of Agricultural Engineering, 13(1):12, 2024.
RAHAYU, S.; RAHMAWATI.; KURNIATUHADI, R. Deteksi bakteri selulolitik pada kotoran luwak (Paradoxurus hermaphroditus) dari Kebun Binatang Bandung. Protobiont, 7(2):19-28, 2018.
SAIDI, B. B.; SURYANI, E. Evaluasi kesesuaian lahan untuk pengembangan kopi liberika di kabupaten tanjung jabung timur jambi. Jurnal Ilmiah Ilmu Terapan Universitas Jambi, 5(1):1-15, 2021.
SAW, A. K. C. et al. A Comparative study of the volatile constituents of southeast asian Coffea arabica, Coffea liberica and Coffea robusta green beans and their antioxidant activities. Journal of Essential Oil-Bearing Plants, 18(1):64-73, 2015.
SCHENKER, S. et al. Pore structure of coffee beans affected by roasting conditions. Journal of Food Science, 65(3):452-457, 2000.
SETYATI, W. A. et al. Enzyme-producing symbiotic bacteria in gastropods and bivalves molluscs: Candidates for bioindustry materials. Biodiversitas, 24(1):20-25, 2023.
SHARMA, R. et al. Microbial fermentation and its role in quality improvement of fermented foods. Fermentation, 6(4):1-20, 2020.
SILABAN, R. et al. Pengaruh jenis kemasan terhadap karakteristik fisikokimia dan sensori kopi arabika gayo dengan metode pengolahan semi basah. Jurnal Teknologi Pangan dan Hasil Pertanian, 18(2):13, 2023.
SIMATUPANG, M.; HERAWATI, D.; YULIANA, D. FTIR-ATR fingerprinting of robusta and arabica coffee fraction and its correlation with their antioxidant activity. Jurnal Teknologi dan Industri Pangan, 34(1):70-85, 2023.
SIPAYUNG, S. M. et al. The effect of fermentation time by bacillus subtilis on the characteristics of soybean sere. Jurnal Ilmu dan Teknologi Pangan (ITEPA), 8(3):226-237, 2019.
SOMPORN, C. et al. Effects of roasting degree on radical scavenging activity, phenolics and volatile compounds of Arabica coffee beans (Coffea arabica L. cv. Catimor). International Journal of Food Science and Technology, 46(11):2287-2296, 2011.
SU, Y. et al. Research on degradation of polysaccharides during Hericium erinaceus fermentation. LWT - Food Science and Technology, 173:114276, 2023.
SUHENDI, A.; ROHMAN, A.; CAHYANINGRUM, S. Validation of method analysis on determination protein level snakehead fish extract by lowry and bromocresol green method. Jurnal Kefarmasian Indonesia, 13(1):50-58, 2023.
TAMANNA, N.; MAHMOOD, N. Food processing and maillard reaction products: Effect on human health and nutrition. International Journal of Food Science, Article ID 526762, 6 pages, 2015.
TARIGAN, E. B. et al. The changes in chemical properties of coffee during roasting: A review. IOP Conference Series: Earth and Environmental Science, 974:012115, 2022.
TARIGAN, I. L. et al. Liberica coffee enriched with Cinnamon (Cinnamomum verum): synergetic study of sensory, antioxidant activity, and chemical components. Coffee Science, 18: e182149, 2023.
WALUYO, E. A.; NURLIA, A. Potential development of agroforestry coffea liberica and its marketing prospect to support peatland restoration of south sumatra (Learning from Tanjung Jabung Barat District, Jambi Province). Potential Development of Agroforestry. p. 255-264, 2017.
WANG, C. et al. Coffee flavour modification through controlled fermentation of green coffee beans by Saccharomyces cerevisiae and Pichia kluyveri: Part II. Mixed cultures with or without lactic acid bacteria. Food Research International, 136:109452, 2020.
WIBOWO, N. A. et al. Effect of fermentation on sensory quality of liberica coffee beans inoculated with bacteria from saliva arctictis binturong raffles, 1821. Biodiversitas, 22(9):3922-3928, 2021.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Coffee Science - ISSN 1984-3909Os direitos autorais dos artigos publicados nesta revista pertencem aos autores, com os primeiros direitos de publicação pertencentes à revista. Como os artigos aparecem nesta revista com acesso aberto, eles podem ser usados livremente, com as devidas atribuições, em aplicativos educacionais e não comerciais.