Inhibition of respiration by light in Coffea arabica


  • Indira Pereira de Oliveira Universidade Federal de Lavras/UFLA, Departamento de Biologia, Setor de Fisiologia Vegetal, Lavras, MG, Brasil.
  • João Paulo Rodrigues Alves Delfino Barbosa Universidade Federal de Lavras/UFLA, Departamento de Biologia, Setor de Fisiologia Vegetal, Lavras, MG, Brasil.
  • Scott Saleska The University of Arizona, Department of Ecology and Evolutionary Biology, Tucson, Arizona, Estados Unidos.
  • João Paulo Pennacchi Universidade Federal de Lavras/UFLA, Departamento de Biologia, Setor de Fisiologia Vegetal, Lavras, MG, Brasil.
  • Neill Prohaska The University of Arizona, Department of Ecology and Evolutionary Biology, Tucson, Arizona, Estados Unidos.



Describing gas exchange between plants and the atmosphere is essential to improve current estimates of fluxes and carbon stocks. Light is directly related to gas exchange; when plants are under low light intensities, there is an increase in photosynthetic quantum yield and, consequently, in respiratory rate. That means there is an apparent inhibition of respiration under high light intensity, a phenomenon known as the Kok effect. Considering the effect of light and leaf age, the aim of this study was to describe the physiological responses of daytime gas exchange related to leaves of different ages in Coffea arabica L. cv. Catuaí Vermelho, seeking to identify leaf respiration inhibition by light. The experiment was conducted at the Universidade Federal de Lavras, Minas Gerais, Brazil. Leaves were measured using an infrared gas analyzer (IRGA; LI- 6400XT, LI-COR, Lincoln, NE, USA) and the fluxes were measured to create a light response curve in “in situ”, thus obtaining photosynthetic parameters, used to estimate light and dark respiration, also the rate of inhibition of leaf respiration by light throughout the leaf age. The assimilation (A_sat) and quantum efficiency did not differ among young, mature, and old leaves, showing that photosynthetic process was not affected by leaf age; however, light and dark respiration were higher in young and mature leaves than in old leaves. Inhibition of respiration by light was similar among leaf ages, assuming the occurrence of inhibition of respiration caused by light (the Kok effect).

Key words: Kok effect; quantum efficiency; gas exchange; leaf age.


ALBERT, L. P. et al. Age-dependent leaf physiology and consequences for crown-scale carbon uptake during the dry season in an Amazon evergreen forest. New Phytologist, 219(3):870-884, 2018.

ARAUJO, W. L. et al. Limitations to photosynthesis in coffee leaves from different canopy positions. Plant Physiology and Biochemistry, 46(10):884-890, 2008.

ATKIN, O. K.; SCHEURWATER, I.; PONS, T. L. High thermal acclimation potential of both photosynthesis and respiration in two lowland Plantago species in contrast to an alpine congeneric. Global Change Biology, 12(3):500-515, 2006.

BJÖRKMAN, O; DEMMIG, B. Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta, 170(4):489-504, 1987.

BOTE, A. D. et al. Analysis of coffee (Coffea arabica L.) performance in relation to radiation level and rate of nitrogen supply II. Uptake and distribution of nitrogen, leaf photosynthesis and first bean yields. European Journal of Agronomy, 92:107-114, 2018.

CROUS, K. Y. et al. Light inhibition of leaf respiration in field‐grown Eucalyptus saligna in whole‐tree chambers under elevated atmospheric CO2 and summer drought. Plant, Cell & Environment, 35(5):966-981, 2012.

DAMATTA, F. M. et al. Ecophysiology of coffee growth and production. Brazilian journal of plant physiology, 19:485-510, 2007.

DAMATTA, F. M. Exploring drought tolerance in coffee: a physiological approach with some insights for plant breeding. Brazilian journal of plant physiology, 16:1-6, 2004.

DUBBERSTEIN, D. et al. Resilient and sensitive key points of the photosynthetic machinery of Coffea spp. to the single and superimposed exposure to severe drought and heat stresses. Frontiers in Plant Science, 11:1049, 2020.

GAUTHIER, P. P. G et al. Is the Kok effect a respiratory phenomenon? Metabolic insight using 13C labeling in Helianthus annuus leaves. New Phytologist, 228(4):1243-1255, 2020.

GHAZALI, M. F. et al. Spectral analysis for phenological studies of leaves and fruits development in coffee plantation in Gunung Puntang: A preliminary result. IOP Conference Series: Earth and Environmental Science, 500:012024, 2020.

GOMES, F. P Experimental statistics. Nobel, 1973.

HESKEL, M. A. et al. Bringing the Kok effect to light: a review on the integration of daytime respiration and net ecosystem exchange. Ecosphere, 4(8):1-14, 2013.

HESKEL, M. A.; TANG, J. Environmental controls on light inhibition of respiration and leaf and canopy daytime carbon exchange in a temperate deciduous forest. Tree physiology, 38(12):1886-1902, 2018.

INTERNATIONAL COFFEE ORGANIZATION - ICO. Sustainability of the coffee sector in Africa. International Coffee Council, London, U. K., Tech. Rep. ICC114-5, 2015.

INTERNATIONAL COFFEE ORGANIZATION - ICO. World coffee consumption. 2020. Available in: Accessed on 10/23/2020 at 12:02. Access in: 13 December 2023.

KEENAN, T. F. et al. Widespread inhibition of daytime ecosystem respiration. Nature ecology & evolution, 3(3):407-415, 2019.

KIRSCHBAUM, M. U.; FARQUHAR, G. D. Investigation of the CO2 dependence of quantum yield and respiration in Eucalyptus pauciflora. Plant Physiology, 83(4):1032-1036, 1987.

KOK, B. On the interrelation of respiration and photosynthesis in green plants. Biochimica et biophysica acta, v. 3, p. 625-631, 1949.

KÖPPEN, W. Climatologia: Com um estudio de los climas de la tierra México. Fundo de Cultura Econômica, 1948. 478p.

MARIAS, D. E.; MEINZER, F. C.; STILL, C. Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica. Ecology and Evolution, 7(4):1297-1310, 2017.

MENDONÇA, R. et al. Approach to biennial production in coffee plants. Biosphere Encyclopedia, 7:13, 2011.

SANTIN, M. R. et al. Yield, maturation cycle, and estimates of genetic parameters of Robusta coffee genotypes under irrigation in the Cerrado. Crop Breeding and Applied Biotechnology, 19(4):387-394, 2019.

SILVA, E. A. et al. Seasonal changes in vegetative growth and photosynthesis of Arabica coffee trees. Field Crops Research, 89(2-3):349-357, 2004.

TCHERKEZ, G. et al. Respiratory metabolism of illuminated leaves depends on CO2 and O2 conditions. Proceedings of the National Academy of Sciences, 105(2):797-802, 2008.

TCHERKEZ, G. et al. Leaf day respiration: Low CO2 flux but high significance for metabolism and carbon balance. New Phytologist, 216(4):986-1001, 2017.

WAY, D. A. et al. Diurnal and seasonal variation in light and dark respiration in field-grown Eucalyptus pauciflora. Tree Physiology, 35(8):840-849, 2015.

WOHLFAHRT, G.; GU, L. The many meanings of gross photosynthesis and their implication for photosynthesis research from leaf to globe. Plant Cell Environ, 38:2500-2507, 2015.

WU, J. et al. Leaf development and demography explain photosynthetic seasonality in Amazon evergreen forests. Science, 351(6276):972-976, 2016.

YIN, X. et al. Evaluating a new method to estimate the rate of leaf respiration in the light by analysis of combined gas exchange and chlorophyll fluorescence measurements. Journal of experimental botany, 62(10):3489-3499, 2011.




How to Cite

OLIVEIRA, I. P. de; BARBOSA, J. P. R. A. D.; SALESKA, S.; PENNACCHI, J. P.; PROHASKA, N. . Inhibition of respiration by light in Coffea arabica. Coffee Science - ISSN 1984-3909, [S. l.], v. 18, p. e182162, 2023. DOI: 10.25186/.v18i.2162. Disponível em: Acesso em: 12 apr. 2024.