Regeneration and development of Coffea arabica L. plants through indirect somatic embryogenesis

Authors

DOI:

https://doi.org/10.25186/.v18i.2078

Abstract

Micropropagation of AS2K clones Arabica coffee (Coffea arabica L.) was attempted through indirect somatic embryogenesis by using ten different parts of the leaf such as shoot, first leaf base, second leaf base, third leaf base, first leaf middle, second leaf middle, third leaf middle, first leaf tip, second leaf tip, and third leaf tip. The influence of the part of leaf explants, combination of plant growth regulator (PGRs) in the induction of embryogenic callus and regeneration of embryo somatic were studied. Furthermore, the various protocols to induce regeneration of somatic embryo into plantlet through different step of subculture and the use of various germination medium were demonstrated. The morphological characteristics and histological analysis of embryogenic callus and embryo development were observed. In this experiment, it was observed that the M5 medium supplemented with 1 mg/L 2,4-D, 1 mg/L BAP and 4 mg/L 2-ip was closely associated with third leaf tip explants for induction of embryogenic callus. The maximum number of globular, heart-shape, torpedo and cotyledones (18, 4, 12, 4, respectively) were achieved on ERM6 medium containing 2 mg/L BAP without activated charcoal on 90th day for regeneration of embryo somatic. The length of roots is the most influence paramater on plantlet regeneration, and the 17th protocol which used B medium, large embryos and twice phase of subculture from liquid medium to solid medium is the best protocol for plantlet regeneration. The protocol developed could be useful highly for large-scale micropropagation in these commercially important Arabica coffee clones.

Key words: Indirect somatic embryogenesis; 24-D; BAP; histology; anatomy.

References

AGUILAR-HERNÁNDEZ, V.; LOYOLA-VARGAS, V. M. Advanced proteomic approaches to elucidate somatic embryogenesis. Frontiers in Plant Science, 9:1658, 2018.

ALMEIDA, J. A. S. et al. Embriogênese somática em genótipos de Coffea arabica L. Coffee Science, 3(2):143-151, 2008.

AWADA, R. et al. An innovative automated active compound screening system allows high-throughput optimization of somatic embryogenesis in Coffea arabica. Scientific Reports, 10:810, 2020.

BARTOS, P. M. C. et al. Histology of somatic embryogenesis in Coffea arabica L. Biologia, 73:1255-1265, 2018.

BERTHOULY, M.; ETIENNE, H. Somatic embryogenesis of coffee. In: JAIN, S. M.; GUPTA, P. K.; NEWTON, R. J. (eds) Somatic embryogenesis in woody plants. Springer, Dordrecht: Forestry Sciences, v 59, p. 259-287, 1999.

BOBADILLA LANDEY, R. et al. High genetic and epigenetic stability in coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by AFLP, MSAP and the phenotypic variation rate. PLoS ONE, 8(2):e56372, 2013.

BUCKSETH, T. et al. Optimization of activated charcoal on in vitro growth and development of potato (Solanum tuberosum L.). International Journal of Current Microbiology and Applied Sciences, 7(10):3543-3548, 2018.

CAMPOS, N. A.; PANIS, B.; CARPENTIER, S. C. Somatic embryogenesis in coffee: The evolution of biotechnology and the integration of omics technologies offer great opportunities. Frontiers in Plant Science, 8:1460, 2017.

DE ALMEIDA, J. A. S. Observations on somatic embryogenesis in Coffea arabica L. In: CASTANHEIRA, D. T. (Ed.). Coffee-production and research. IntechOpen, p. 1-20, 2020.

ARIMARSETIOWATI, R. et al. ETIENNE, H. et al. Coffee somatic embryogenesis: How did research, experience gained and innovations promote the commercial propagation of elite clones from the two cultivated species? Frontiers in Plant Science, 9:1630,

ETIENNE, H. et al. Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects. Tree Physiology, 33(6):640-653, 2013.

FEHÉR, A.; PASTERNAK, T. P.; DUDITS, D. Transition of somatic plant cells to an embryogenic state. Plant Cell, Tissue and Organ Culture, 74:201-228, 2003.

FITRIANA, D. et al. Effect of combination explant difference leaf part and concentration of active charcoal on callus initiation mangrove (Rhizophora apiculata BI) by in-vitro. Journal of Physics: Conference Series, 1217:012166, 2019.

FREITAS, N. C. et al. In silico and in vivo analysis of ABI3 and VAL2 genes during somatic embryogenesis of Coffea

arabica: Competence acquisition and developmental marker genes. Plant Cell, Tissue and Organ Culture, 137:599-611, 2019.

GATICA, A. M.; ARRIETA, G.; ESPINOZA, A. M. Comparison of three in vitro protocols for direct somatic embryogenesis and plant regeneration of Coffea arabica L. CVS. Caturra and Catuaí. Agronomia Costarricense, 31(1):85-94, 2007.

GEORGET, F. et al. Somatic embryogenesis-derived coffee plantlets can be efficiently propagated by horticultural

rooted mini-cuttings: A boost for somatic embryogenesis. Scientia Horticulturae, 216:177-185, 2017.

GUAN, Y. et al. Application of somatic embryogenesis in woody plants. Frontiers in Plant Science, 7:938, 2016.

HATANAKA, T. et al. Effect of plant growth regulators on somatic embryogenesis in leaf cultures of Coffea

canephora. Plant Cell Reports, 10:179-182, 1991.

HULUPI, R. Panduan determinasi varietas dan klon kopi Indonesia berdasarkan sifat morfologi. Pusat Penelitian Kopi dan Kakao Indonesia. 2016. Available in: https://iccri.net. Access in: February 7, 2023.

JIMÉNEZ, V. M. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulation, 47:91-110, 2005.

KALVE, S.; DE VOS, D.; BEEMSTER, G. T. S. Leaf development: A cellular perspective. Frontiers in Plant Science, 5:362, 2014.

KARAMI, O.; AGHAVAISI, B.; MAHMOUDI, P. A. Molecular aspects of somatic-to-embryogenic transition in plants. Journal of Chemical Biology, 2(4):177-190, 2009.

KARLSSON, S. B.; VASIL, I. K. Morphology and ultrastructure of embryogenic cellsuspension cultures of panicum maximum (Guinea grass) and pennisetumpurpureum (Napier grass). American Journal of Botany, 73(6):894-901, 1986.

KIEBER, J. J.; SCHALLER, G. E. Cytokinins. Arabidopsis Book, 12:e0168, 2014.

KUMARI, A.; BASKARAN, P.; VAN STADEN, J. In vitro propagation via organogenesis and embryogenesis of cyrtanthus mackenii: A valuable threatened medicinal plant. Plant Cell, Tissue, and Organ Culture, 131(3):407-415, 2017.

LEE, Y. et al. Influence of auxins, cytokinins, and nitrogen on production of rutin from callus and adventitious roots

of the white mulberry tree (Morus alba L.). Plant Cell, Tissue, and Organ Culture, 105:9-19, 2011.

LOYOLA-VARGAS, V. M. et al. Somatic embryogenesis in Coffea spp. In: LOYOLA-VARGAS, V. M.; OCHOAALEJO,

N. Somatic embryogenesis: Fundamental aspects and applications. New York: ed. Springer, p. 241-266, 2016.

MISHRA, M. K.; SLATER, A. Recent advances in the genetic transformation of coffee. Biotechnology Research

International, Article ID 580857, p. 1-17, 2012.

MOLINA, D. M. et al. The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell,

Tissue and Organ Culture, 71:117-123, 2002.

NAVARRO, B. V. et al. Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the

endangered conifer, Araucaria angustifolia. PLoS ONE, 12(7):e0180051, 2017.

NIC-CAN, G. I. et al. Somatic embryogenesis: Identified factors that lead to embryogenic repression. A case of species of the

same genus. PLoS ONE, 10(6):e0126414, 2015.

NIC-CAN, G. I.; LOYOLA-VARGAS, V. M. The role of the auxins during somatic embryogenesis. In: LOYOLAVARGAS,

V.; OCHOA-ALEJO, N. (eds) Somatic embryogenesis: Fundamental aspects and applications. Cham: Springer, p. 171-182, 2016.

OGUTU, C. et al. Comprehensive analysis of quality characteristics in main commercial coffee varieties and wild Arabica in Kenya. Food Chemistry: X, 14:100294, 2022.

PRADO, K.; MAUREL, C. Regulation of leaf hydraulics: From molecular to whole plant levels. Frontiers in Plant Science, 4:255, 2013.

QUIROZ-FIGUEROA, F. et al. Histological studies on the developmental stages and differentiation oftwo different somatic embryogenesis systems of Coffea arabica. Plant Cell Reports, 20:1141-1149, 2002.

RUZIN, S. E. Plant microtechnique and microscopy. Oxford, New York: Oxford University Press. Annals of Botany, 86(3):708, 1999.

SALAÜN, C.; LEPINIEC, L.; DUBREUCQ, B. Genetic and molecular control of somatic embryogenesis. Plants, 10(7):1467, 2021.

SILVA, M. C. et al. Coffee resistance to the main diseases: Leaf rust and coffee berry disease. Brazilian Journal of

Plant Physiology, 18(1):119-147, 2006.

SU, Y. H. et al. Plant cell totipotency: Insights into cellular reprogramming. Journal of Integrative Plant Biology,

(1):228-243, 2020.

VALENCIA-LOZANO, E. et al. Development of an efficient protocol to obtain transgenic coffee, Coffea arabica L., expressing the Cry10Aa toxin of Bacillus thuringiensis. International Journal of Molecular Sciences, 20(21):5334, 2019.

VALENCIA-LOZANO, E. et al. Osmotic stressinduced somatic embryo maturation of coffee Coffea arabica L., shoot and root apical meristems development and robustness. Scientific Reports, 11:9661, 2021.

VAN BOXTEL, J.; BERTHOULY, M. High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell, Tissue and Organ Culture, 44:7-17, 1996.

VON ARNOLD, S. et al. Developmental pathways of somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 69:233-249, 2002.

WIJAYATI, A.; SOLICHATUN, D. S. Pengruh asam indol asetat terhadap pertumbuhan dan diamteter sel sekretori rimpang tanaman kunyit (Curcuma domestica Val.). Biofarmasi, 3(1):16 -21, 2005.

WILLIAMS, E. G.; MAHESWARAN, G. Somatic embryogenesis: Factors influencing coordinated behaviour of cells as an embryogenic group. Annals of Botany, 57(4):443-462, 1986.

XIE, D. Y.; HONG, Y. Regeneration of acacia mangium through somatic embryogenesis. Plant Cell Reports, 20:34-40, 2001.

ŻUR, I. et al. Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures. Plant Cell Reports, 34:47-62, 2015.

ZÜRCHER, E.; MÜLLER, B. Cytokinin synthesis, signaling, and function- advances and new insights. International Review of Cell and Molecular Biology, 324:1-38, 2016.

Downloads

Published

2023-03-10

How to Cite

ARIMARSETIOWATI, R. .; DARYONO, B. S. .; ASTUTI, Y. T. M.; PRASTOWO, E. .; SEMIARTI, E. Regeneration and development of Coffea arabica L. plants through indirect somatic embryogenesis. Coffee Science - ISSN 1984-3909, [S. l.], v. 18, p. e182078, 2023. DOI: 10.25186/.v18i.2078. Disponível em: https://coffeescience.ufla.br/index.php/Coffeescience/article/view/2078. Acesso em: 12 apr. 2024.