Characterization of active-aroma wheel in contemporary coffee processes via gas chromatography–olfactometry, and sensory perspective


  • Wanphen Jitjaroen Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang. Lampang, Thailand.
  • Daorung Chaisri Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang. Lampang, Thailand.
  • Lachinee Panjai Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang. Lampang, Thailand.



This research is to study the difference in chemical changes during fermentation, between the new fermentation processes. Aroma descriptors and sensorial assessments can be effectively used to tailor made fermentation processes. Coffee cherries (Coffea arabica L. var. Catimor) were treated with three different processes as followed: 1) Dry process (control), 2) Semi-carbonic maceration process (SCM): Carbon dioxide gas was injected to replace oxygen, and 3) Yeast process: coffee cherries were fermented by commercial yeast strain Saccharomyces cerevisiae var cerevisiae. SCM and Yeast processes were both incubated at 17±1oC until mucilage of pulped coffee and pectin layer reached to 4.2-4.3 of pH value. Low air temperatures (20-33 oC), low relative humidity (25-60%) to dry coffee samples to the desired 12.5% moisture content was used. The chemical component of the intact mucilage during fermentation
was analyzed. The active-aroma attributes of roasted coffee were qualified and intensified by gas chromatography–olfactometry, categorized as followed: Enzymatic, Sugar browning, Dry distillation and Aromatic, and translated into an active-aroma wheel. The quality cup scores were evaluated by certified Q arabica graders, according to the standard of the Specialty Coffee Association. Results shows that, when compared to Control, SCM and Yeast process had a greater potential when it comes to increasing active-aroma attributes (twenty, twenty-nine, and twenty-two active-aroma attributes respectively). The fermentation process of SCM and Yeast process changes the post fermentation chemical composition of coffee cherry, a decrease in pH value, and an increase in acidity and ethanol. Both processes resulted in an improvement in aromatic attributes of roasted coffee, in both types and intensities. In line with the cup quality’s final scores of 81.50, and 82.83 (specialty coffee), respectively, both processes scored higher than the Dry process
(79.42 cup score), with coffee from Yeast process scoring the highest in significant difference.

Key words: Coffee; active-aroma wheel; semi-carbonic maceration process; yeast process; gas chromatography-olfactometry.


ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS - AOAC. Official methods of analysis. V.69. No. 2. 17th ed. Gaithersburg, Md, 2000. 233p.

AVALLONE, S. et al. Microbiological and biochemical study of coffee fermentation. Current Microbiology, 42:252-256, 2001.

BANDARA, D. Understanding The Coffee Tasters Flavour wheel, Coffee perception, 2014. Available at: Accessed

on: March, 14, 2022.

BERTRAND, B. et al. Comparison of bean biochemical composition and beverage quality of Arabica hybrids involving Sudanese Ethiopian origins with traditional varieties at various elevations in Central America. Tree Physiology, 26(9):1239-1248, 2006.

BRESSANI, A. P. P. et al. Infuence of yeast inoculation on the quality of fermented coffee (Coffea arabica var. Mundo Novo) processed by natural and pulped natural processes. International Journal of Food Microbiology, 343:109107, 2021.

BRESSANI, A. P. P. et al. Organic acids produced during fermentation and sensory perception in specialty coffee using yeast starter culture. Food Research International, 128:108773, 2020.

BRIOSCHI, J. D. et al. Microbial fermentation affects sensorial, chemical, and microbial profile of coffee under carbonic maceration. Food Chemistry, 342:128296, 2020.

CAPELLI, L. et al. Review on odor pollution, odor measurement, abatement techniques, D-NOSES, H2020-SwafS-23-2017-789315, 2019. 80p.

CHINDAPAN, N.; SOYDOK, S.; DEVAHASTIN, S. Roasting kinetics and chemical composition changes of robusta coffee beans during hot air and superheated steam roasting. Journal of Food Science, 84:292-302, 2019.

DE BRUYN, F. et al. Exploring the impacts of postharvest processing on the microbiota and metabolite profiles during green coffee bean production. Applied and Environmental Microbiology, 83:16, 2017.

DE PEREIRA, M. et al. Exploring the impacts of postharvest processing on the aroma formation of coffee beans: A review, Food Chemistry, 272: 441-452, 2019.

DELAHUNTY, C. M.; EYRES, G.; DUFOUR, J. P. Gas chromatography-olfactometry. Journal of Separation Science, 29(14):2107-2125, 2006.

Coffee Science, 18:e182059, 2023.

ETAIO, I. et al. Dynamic sensory description of rioja alavesa red wines made by different winemaking practices by using temporal dominance of sensations. Journal of the Science of Food and Agriculture, 96(10):3492-3499, 2016.

ETHIOPIAN COMMODITY EXCHANGE. Coffee contracts: September. Addis Ababa, 2015. 14p.

EVANGELISTA, S. R. et al. Improvement of coffee beverage quality by using selected yeasts strains during the fermentation in dry process. International Food Research Journal, 61:183-195, 2014.

EVANGELISTA, S. R. et al. Microbiological diversity associated with the spontaneous wet method of coffee fermentation. International Journal of Food Microbiology, 210:102-112, 2015.

FUNDIRA, M.; DUEZ, C.; SIECZKOWSKI, N. Coffee fermentation back to basics, the role of yeast in coffee processing. African Fine Coffees Review Magazine, v.10(2): p. 32-33, 2020.

GUDI, P. Carbonic maceration (A unique way of coffee processing). Maceracion, 1-4, 2017.

GUNES, G.; BLUM, L. K.; HOTCHKISS, J. H. Inactivation of yeasts in grape juice using a continuous dense phase carbon dioxide processing system. Journal of the Science of Food and Agriculture, 85(14):2362-2368, 2005.

GUZZON, R. et al. The impact of grape processing and carbonic maceration on the microbiota of early stages of winemaking. Journal of Applied Microbiology, 128:209-224, 2020.

HAILE, M.; KANG, W. H. Isolation, identification, and characterization of pectinolytic yeasts for starter culture in coffee fermentation. Microorganisms, 7(10):401, 2019.

HAILE, M.; KANG, W. H. The harvest and post-harvest management practices’ impact on coffee quality. In: CASTANHEIRA, D. T. Coffee, 2019. 170p.

JITJAROEN, W. Coffivino process: A new era of coffee fermentation. Journal of Science and Agricultural Technology, 2(2):1-5, 2021.

JITJAROEN, W.; BOUPHUN, T.; PANJAI, L. The potential of malolactic fermentation on organic acids degradation in mao (Antidesma Thwaitesanum Müell.) wine production. International Journal of Bioscience, Biochemistry and Bioinformatics, 3(4):368-371, 2013.

KWAK, H. S.; JEONG, Y.; KIM. M. Effect of yeast fermentation of green coffee beans on antioxidant activity and consumer acceptability. Journal of Food Quality, 3:1-8, 2018.

LAI, Y.-T. et al. Isolation and identification of aroma producing strain with esterification capacity from yellow water. Plos One, 14(2):1-16, 2019.

LINGLE, T. R. The coffee cupper’s handbook: Systematic guide to the sensory evaluation of coffee’s Flavor, 4th ed. Specialty Coffee Association of America. Long Beach: CA, USA. 2011. 66p.

LIU, L. et al. Effect of carbonic maceration pre-treatment on drying kinetics of chilli (Capsicum annuum L.) flesh and quality of dried product. Food Bioprocess Technology, 7(9):2516-2527, 2014.

MARTINEZ, S. J. et al. Different inoculation methods for semidry processed coffee using yeasts as starter cultures. Food Research International, 102:333-340, 2017.

MARTINEZ, S. J. et al. The altitude of coffee cultivation causes shifts in the microbial community assembly and biochemical

compounds in natural induced anaerobic fermentations. Frontiers in Microbiology, 12:671395, 2021.

MEENAKSHI, A.; JAGAN, L. An impression of coffee carbohydrates. Food Science and Nutrition, 47(1):51-67, 2007.

NAVARRO, S. et al. Evolution of chlorpyrifos, fenarimol, metalaxyl, penconazole, and vinclozolin in red wines elaborated by carbonic maceration of monastrell grapes. Journal of Agricultural and Food Chemistry, 48:3537-3541, 2000.

NIU, Y. et al. Characterization of the major aroma-active compounds in peach (Prunus persica L. Batsch) by gas chromatography–olfactometry, flame photometric detection and molecular sensory science approaches. Food Research International, 147:110457, 2021.

OHLOFF, G. The chemistry of organic natural products. In: HERZ, W.; GRISEBACH, H.; KIRBY, G. W. Springer-Verlag. New York, p. 73-132, 1978.

ONDIER, G. O.; SIEBENMORGEN, T. J.; MAUROMOUSTAKOS, A. Low-temperature, lowrelative humidity drying of rough rice. Journal of Food Engineering, 100(3):545-550, 2010.

PETER, K.; ONG, C.; TERRY, E. Gas chromatography/ olfactory analysis of lychee (Litchi chinesis Sonn.). Journal of Agricultural and Food Chemistry, 46(6):2282-2286, 1998.

PEREIRA, G. V. M. et al. Conducting starter culturecontrolled fermentations of coffee beans during on-farm wet processing: Growth, metabolic analyses and sensorial effects. International Food Research Journal, 75:348-356, 2015.

PEREIRA, G. V. M. et al. Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. International Journal of Food Microbiology, 188:60-66, 2014.

PEREIRA, G. V. M. et al. Microbial ecology and starter culture technology in coffee processing. Critical Reviews in Food Science and Nutrition, 57:2775-2788, 2017.

PEREIRA, L. L. et al. New propositions about coffee wet processing: Chemical and sensory perspectives. Food Chemistry, 310:125943, 2020.

PEREIRA, P. V. et al. Microbial diversity and chemical characteristics of Cofea canephora grown in different environments and processed by dry method. World Journal of Microbiology and Biotechnology, 37(51):1-12, 2021.

RETA. et al. Reducing the acidity of arabica coffee beans by ohmic fermentation technology. Food Research, 1(5):157-160, 2017.

RIBEIRO, L. S. et al. Behavior of yeast inoculated during semi-dry coffee fermentation and the effect on chemical and sensorial properties of the final beverage. Food Research International, 92:26-32, 2017.

RITTHIRUANGDEJ, P. Data analysis for research and product development using SPSS. Vista Inter Print, 2018. 375p.

ROBINSON, J. The oxford companion to wine. 3rd. Ed. Oxford University Press. p. 268-780, 2006.

SILVA, C. F. Microbial activity during coffee fermentation. In: SCHWAN, R. F.; FLEET, G. H. Cocoa and Coffee Fermentations; Boca Raton, Florida: CRC Press, p. 368-423, 2014.

SPECIALTY COFFEE ASSOCIATION - SCA. Protocol & best practice. 2021. Available in: <> Access in: August, 22, 2021.

SUNARHARUM, W. B.; WILLIAMS, D. J.; SMYTH, H. E. Complexity of coffee flavor: A compositional and sensory perspective. Food Research

International, 62:315-325, 2014.

TESNIERE, C.; FLANZY, C. Carbonic maceration wines: Characteristics and winemaking process. Advances in Food and Nutrition Research, 63:1-15, 2011.

VAAST, P. et al. Fruit thinning and shade improve bean characteristics and beverage quality of coffee (Coffea arabica L.) under optimal conditions. Journal of the Science of Food and Agriculture, 86(2):197-204, 2006.

VARELA, P.; ARES, G. Sensory profiling, the blurred line between sensory and consumer science. A review of novel methods for product characterization. Food Research International, 48(2):893-908, 2012.

VELOSO, T. G. R. et al. Efects of environmental factors on microbiota of fruits and soil of Cofea arabica in Brazil.

Scientific Report, 10:14692, 2020.

ZHANG, Y. S. et al. The effect of carbonic maceration during winemaking on the color, aroma and sensory properties of

‘muscat hamburg’ wine. Molecules, 24:3120, 2019.




How to Cite

JITJAROEN, W.; CHAISRI, D.; PANJAI, L. Characterization of active-aroma wheel in contemporary coffee processes via gas chromatography–olfactometry, and sensory perspective. Coffee Science - ISSN 1984-3909, [S. l.], v. 18, p. e182059, 2023. DOI: 10.25186/.v18i.2059. Disponível em: Acesso em: 12 apr. 2024.