Alternative treatments diminish oviposition and viability of Leucoptera coffeella (Lepidoptera: Lyonetiidae) eggs and larvae on coffee plants


  • Humberto Godoy Androcioli Instituto de Desenvolvimento Rural do Paraná/IAPAR-EMATER, Departamento de Entomologia, Londrina, PR, Brasil.
  • Adriano Thibes Hoshino Universidade Estadual de Londrina/UEL, Departamento de Ciências Agrárias, Londrina, PR, Brasil.
  • Ana Maria Meneghin Instituto de Desenvolvimento Rural do Paraná/IAPAR-EMATER, Departamento de Entomologia, Londrina, PR, Brasil.
  • Mauricio Ursi Ventura Universidade Estadual de Londrina/UEL, Departamento de Ciências Agrárias, Londrina, PR, Brasil.
  • Bruna Aparecida Guide Instituto de Desenvolvimento Rural do Paraná/IAPAR-EMATER, Departamento de Entomologia, Londrina, PR, Brasil.
  • Fernando Teruhiko Hata Universidade Estadual de Londrina/UEL, Departamento de Ciências Agrárias, Londrina, PR, Brasil.
  • Ayres de Oliveira Menezes Junior Universidade Estadual de Londrina/UEL, Departamento de Ciências Agrárias, Londrina, PR, Brasil.



The effects of alternative inputs on the oviposition and viability of Leucoptera coffeella eggs and larvae were evaluated. Under controlled conditions, coffee seedlings cv. IAPAR-59, eight months old, were sprayed with brown propolis extract (1%), pyroligneous extract with pepper and garlic (PEPG) (2%), silicate clay (2%), kaolin (5%), lime sulfur (2%), neem oil (1%) and kaolin + neem oil (5% + 1%), distilled water and no apraying. In a first no-choice bioassay, coffee seedlings were sprayed before oviposition and kept in cages where adult insects within three days after emergence were released. Adults remained in the cages for 24 hours. Eggs were then recorded. A portion of these eggs were removed but 10 eggs per seedling were preserved to access larval mortality. Reduction of the number of eggs by propolis extract, neem oil, kaolin + neem oil and PEPG were observed in the evaluations. Treatments with neem oil caused great larval inviability. Treatments were also sprayed on eggs laid on leaves. Eggs viability was reduced by neem oil containing treatments and lime sulfur. Neem oil treatments made unfeasible adult emergence; intermediate viability on lime sulfur and slight inviability on silicate clay. Finally, treatments were also sprayed on leaves hosting first or third instar larvae. Neem treatment caused high mortality for 1st and 3rd larvae which was reduced when mixed with kaolin. However, these negative effects disappeared when considering adult survival.   Results indicated that propolis extract, PEPG and neem oil treatment are suitable for reducing egg deposition and neem oil diminishes markedly larvae survival and adult emergence.

Author Biography

Humberto Godoy Androcioli, Instituto de Desenvolvimento Rural do Paraná/IAPAR-EMATER, Departamento de Entomologia, Londrina, PR, Brasil.

Possui graduação em Engenharia Agronomica pela Universidade Estadual de Londrina (2005), mestrado em (2008) e doutor em (2010) em Agronomia pela Universidade Estadual de Londrina. Tem experiência na área de Agronomia, com ênfase em Entomologia Agrícola, atuando como pesquisador Entomologista do Instituto Agronômico do Paraná des de 2012.


ABBOTT, C.E. The toxic gases of lime-sulfur. Journal of Economic Entomology, 38:618-620, 1945.

AFONSO, A.P.S et al. Avaliação da calda sulfocálcica e do óleo mineral no controle da cochonilha-parda Parthenolecanium persicae (Hemiptera: Coccidae) na cultura da videira. Arquivos Instituto Biológico, 74(2):167-169, 2007.

ALVES, M. et al. Ação de diferentes preparações de extrato pirolenhoso sobre Brevipalpus phoenicis (GEIJSKES). Revista Brasileira de Fruticultura, 29(2):382-385, 2007.

ATTIA, S. et al. Acaricidal activity of 31 essential oils extracted from plants collected in Tunisia. Journal of Essential Oil Research, 24(3):279-288, 2012.

AYRES, M. et al. BioEstat 5.0: Aplicações estatísticas nas áreas das ciências biológicas e médicas. 5ª ed. Belém: Sociedade Civil Mamirauá, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasília, 2007. 380p.

AZEVEDO, F. R. DE. et al. Eficiência de produtos naturais para o controle de Bemisia tabaci biotipo B (Hemiptera: Aleyrodidae) em meloeiro. Arquivos do Instituto Biológico, 72(1):73-79, 2005.

BANKOVA, V.S.; DE CASTRO, S.L.; MARCUCCI, M. C. Propolis: Recent advances in chemistry and plant origin. Apidologie, 31:3-15, 2000.

BLANEY, W. M.; SIMMONDS, M. S. J. A behavioural and electrophysiological study of the role of tarsal chemoreceptors in feeding by adults of Spodoptera littoralis, Heliothis virescens and Helicoverpa armigera. Journalof Insect Physiology, 36:743-756, 1990.

BENELLI, G. et al. Neem (Azadirachta indica): Towards the ideal insecticide?. Natural Product Research, 31(4):369-386, 2016.

BOONGIRD, S. Honey and non-honey foods from bees in Thailand. In: DURST, P.B et al. Forest insects as food: Humans bite back. Food and agriculture organization of the united nations regional office for Asia and the Pacific, p.165-172, 2010.

BOTTI, J.M.C. Controle alternativo do Brevicoryne brassicae (Hemiptera: Aphididae) com extratos de diferentes espécies de plantas. Revista Brasileira de Ciências Agrárias, 10(2):178-183, 2015.

CANTERI, M. G. et al. SASM - Agri: Sistema para análise e separação de médias em experimentos agrícolas pelos métodos Scott-Knott, Tukey e Duncan. Revista Brasileira de Agrocomputação, 1(2):18-24, 2001.

CONSELHO DOS EXPORTADORES DE CAFÉ DO BRASIL - CECAFÉ. Exportações brasileiras de café.2020. Available in: <>. Access in: 25,November, 2021.

CHANDRAMOHAN, N.; NAMJA, N. Effect of plant product spray on the ovipositional behaviour of diamond back moth, Plutella xylostella (L.). NeemNewsletter,9(1):8-9, 1992.

CHAUDHARY, S. et al. Progress on Azadirachta indica based biopesticides in replacing synthetic toxic pesticides. Frontiers In Plant Science, 8:610, 2017.

CIRASINO, L.; PISATI, A.; FASANI, F. Contact dermatitis from propolis. Contact Dermatitis, 16:110-111, 1987.

COELHO JUNIOR, A.; DESCHAMPS, F.C. Ação sistêmica e translaminar do óleo de nim visando ao controle de Tuta absoluta (Meyrick)(Lep.: Gelechiidae) em tomateiro. Arquivos do Instituto Biológico, 81(2):140-144, 2014.

COMPANHIA NACIONAL DE ABASTECIMENTO - CONAB. Acompanhamento da safra brasileira de café - Safra 2020 – Primeiro Levantamento. CONAB, Brasília - DF. 2020. Available in: <>. Access in: May, 17, 2021.

DAMIANI, N. et al. Evaluation of the toxicity of a propolis extract on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae). Journal of Apicultural Research, 49(3):257-264, 2010.

DAVID-RUEDA, G. et al. Diagnóstico de Leucoptera coffeella (Lepidoptera: Lyonitiidae) y sus parasitoides en el departamento de Antioquia, Colombia. Revista Colombiana de Entomologia,42(1):4-11, 2016.

DUANGPHAKDEE, O. et al. Ant repellent resins of honeybees and stingless bees. Insectes Sociaux, 56:333, 2009.

EROGLU, N.; EMEKCI, M.; ATHANASSIOU, C.G. Applications of natural zeolites on agriculture and food production. Journal of the Science of Food and Agriculture, 97(11):3487-3499, 2017.

FANELA, T.L.M. et al. Assessing potential plants extracts to reduce Leucoptera coffeella (lepidoptera: Lyonetiidae) attack in coffee. Coffee Science, 15:e151653, 2020.

FENING, K.O. et al. Sustainable management of two key pests of cabbage, Brassica oleracea var. capitata L.(Brassicaceae), using homemade extracts from garlic and hot pepper. Organic agriculture, 3:163-173, 2013.

GAJMER, T.R. et al. Effect of methanolic extracts of neem (Azadiractha indica A. Juss) and bakain (Melia azedarach L.) seeds on oviposition and egg hatching of Eiras vitella (Fab.) (Lep., Noctuidae). Journal of Applied Entomology, 126(5):238-243, 2002.

GAREDEW, A. et al. Microcalorimetric toxicity investigation of propolis on Tenebrio molitor L. (Coleoptera: Tenebrionidae). Thermochimica Acta, 394(1-2):239-245, 2002.

GOMES, F.H.T. et al. Atividade inseticida de extratos vegetais sobre o pulgão-preto do feijoeiro. Revista de Ciências Agroambientais,15(1):43-52, 2017.

GONÇALVES, S.G. et al. Oviposição do curuquerê e alimentação de suas lagartas neonatas em algodoeiros tratados com caulim. Pesquisa Agropecuária Brasileira, 50(7):526-533, 2015.

HATA, F. T. et al. Plant acceptance for oviposition of Tetranychus urticae on strawberry leaves is influenced by aromatic plants in laboratory and greenhouse intercropping experiments. Agronomy, 10(2):193, 2020.

ISMAN, M. B. Botanical insecticides in the twenty-first century - fulfilling their promise?. Annual Review of Entomology, 65:233-249, 2020.

SHAH, M.A.; KHAN, A.A. Use of diatomaceous earth for the management of stored-product pests. International Journal of Pest Management, 60(2):100-113, 2014.

LEITE, S. A. et al. Profile of coffee crops and management of the Neotropical coffee leaf miner, Leucoptera coffeella. Sustainability, 12(19):8011, 2020.

LIŠKA, A. et al. Efficacy of nine croatian inert dusts against rice weevil Sitophilus oryzae L.(Coleoptera: Curculionidae) on wheat. Emirates Journal of Food and Agriculture, 29(7):485-494, 2017.

MARTINEZ, S.S.; MENEGUIM, A.M. Redução da oviposição e da sobrevivência de ovos de Leucoptera coffeella causadas pelo óleo emocionável de Nim. Manejo integrado de Plagas y Agroecologia, 67:58-62, 2003.

MARTINEZ, S.S.; VAN EMDEN, H.F. Growth disruption, abnormalities and mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) caused by Azadirachtin. Neotropical Entomology, 30(1):113-124, 2001

MEEMKEN, E. M. Do smallholder farmers benefit from sustainability standards? A systematic review and meta-analysis. Global Food Security, 26:100373, 2020.

MMOJIEJE, J.; HORNUNG, A. The potential application of pyroligneous acid in the UK agricultural industry. Journal of Crop Improvement, 29(2):228-246, 2015.

MIKAMI, A.Y. et al. Controle do caruncho-do-feijão Zabrotes subfasciatus com caulim. Ciência Rural, 40(7):1497-1501, 2010.

MOBKI, M. et al. Toxicity and repellency of garlic (Allium sativum L.) extract grown in Iran against Tribolium castaneum (Herbst) larvae and adults. Archives of Phytopathology and Plant Protection, 47(1):59-68, 2014.

PENTEADO, S.R. Controle alternativo de pragas e doenças com as caldas bordalesa, sulfocálcica e Viçosa. Campinas: Buena Mendes Gráfica e Editora, 2000. 95p.

ROCHA, A.M.S.; SANTOS, A.J.S.; GOLÇALVES-GERVÁRIO, R.C.R. Bioatividade de extratos de própolis sobre o pulgão Myzuz persicae Sulzer (Hemiptera: Aphididae) em couve manteiga. Revista de Ciências Agroveterinárias, 16(3):332-337, 2017.

SANTOS, J.H.R. dos. et al. Controle alternativo de pragas e doenças.Fortaleza: UFC, 1988. 216p.

SILVA, A.C.B. et al. Lethal and sublethal effects of neem oil to the predatory mite Proprioseiopsis neotropicus (Acari: Phytoseiidae). Revista Colombiana de Entomología, 39: 221-225, 2013.

STÄDLER, E.; REIFENRATH, E. K. Glucosinolates on the leaf surface perceived by insect herbivores: Review of ambiguous results and new investigations. Phytochemistry Reviews, 8:207-225, 2009.

SOUZA, J. R. et al. Susceptibility of Chrysoperla externa (Hagen, 1861) (Neuroptera: Crysopidae) to insecticides used in coffee crops. Ecotoxicology, 29:1306-1314, 2020.

TAIZ, L. et al. Fisiologia e desenvolvimento vegetal.6ª Ed. Porto Alegre- RS: Artmed, 2017. 888p.

TUELHER, E.S. et al. Toxicity of organic-coffee-approved products to the southern red mite Oligonychus ilicis and to its predator Iphiseiodes zuluagai. Crop Protection, 55:28-34, 2014.

WEINTRAUB, P.G.; HOROWITZ, A.R. Systemic effects of a neem insecticide on Liriomyza huidobrensis Larvae. Phytoparasitica,25(4):283-289, 1997.

WILLER, H. et al. The world of organic agriculture statistics and emerging trends 2021. Research institute of organic agriculture FiBL, IFOAM - Organic International. Bonn - Germany, 2021.340p.

VENZON, M. et al. The potential of NeemAzal for the control of coffee leaf pests. Crop Protection, 24(3):213-219, 2005.

VERKERK, R.H.J.; WRIGHT, D.J. Biological activity of neem seed kernel extracts and synthetic azadirachtin against larvae of Plutella xylostella L. Pesticide Science, 37(1):83-91, 1993.

VERONEZ, B.; SATO, M. E.; NICASTRO, R. L. Toxicidade de compostos sintéticos e naturais sobre Tetranychus urticae e o predador Phytoseiulus macropilis. Pesquisa Agropecuária Brasileira, 47(4):511-518, 2012.




How to Cite

ANDROCIOLI, H. G.; HOSHINO, A. T. .; MENEGHIN, A. M. .; VENTURA, M. U. .; GUIDE, B. A.; HATA, F. T. .; MENEZES JUNIOR, A. de O. . Alternative treatments diminish oviposition and viability of Leucoptera coffeella (Lepidoptera: Lyonetiidae) eggs and larvae on coffee plants. Coffee Science - ISSN 1984-3909, [S. l.], v. 16, p. e161936, 2021. DOI: 10.25186/.v16i.1936. Disponível em: Acesso em: 7 dec. 2023.