Extraction and Purification of Xylooligosaccharides from Coffee Peel Using Endo-β-1,4-D-Xylanase XynBTN63D and Adsorption for Prebiotics
DOI:
https://doi.org/10.25186/.v20i.2354Abstract
This research aims to extract and purify xylooligosaccharides (XOS) from coffee peel xylan using recombinant endo-β-1,4-D-xylanase (XynBTN63D) and adsorption-based methods, and to assess their prebiotic potential. The xylan was hydrolyzed with XynBTN63D at 40°C for 24 hours. The hydrolysate was purified using activated carbon with ethanol (10%–50%) as the eluent, followed by column chromatography. XOS composition was analyzed before and after purification using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Both pre- and post-purification products contained xylobiose (X2) and xylotriose (X3), while ethanol concentrations of 10%, 20%, and 30% were effective in removing residual xylan. Higher ethanol concentrations were less effective in XOS recovery. Activated carbon significantly improved the purity by eliminating impurities and enhancing X2 and X3
separation. The results confirm that the enzyme operates with high specificity, producing oligosaccharides with potential applications in prebiotics and functional food industries. This study demonstrates the potential of coffee peel a widely available agricultural waste as a low-cost, sustainable lignocellulosic source for prebiotic XOS production. The unique application of XynBTN63D in combination with optimized ethanol-based adsorption contributes to the development of efficient XOS purification protocols.
Key words: Prebiotic; enzymatic hydrolysis; bioconversion; purification; lignocellulosic biomass.
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