Enzymes activity as potential molecular markers for drying tolerance and quality in Coffea arabica seeds
DOI:
https://doi.org/10.25186/.v19i.2229Abstract
Antioxidant enzyme expression plays a vital role in the stress response in plants. This study evaluated the physiological quality and antioxidant enzyme expression of coffee seeds and plants subjected to drying. Coffee seeds were conventionally dried to 40% and 10% water content, representing nonstressed and stressed conditions, respectively. The tests included germination, accelerated aging, and emergence. The expression of enzymes (SOD, CAT, POX, APX, and 1-cys-prx), together with lipid peroxidation, was analysed. The results showed no significant differences in the physiological test results, except for germination in stressed seeds. We found differences in antioxidant system enzymes according to the condition applied to the seeds; however, only for peroxidase, lipid peroxidation, and 1-cys-prx were the observed patterns more strongly correlated with seed germination. Nevertheless, only lipid peroxidation and 1-cys-prx were more strongly correlated with germination, indicating its potential as a marker for coffee seed quality.
Key words: Coffee; drought; oxidative stress; 1-cys-prx.
References
AALENF, R. B. et al. Transcripts encoding an oleosin and a dormancy‐related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds. The Plant Journal, 5(3):385-396, 1994.
ALFENAS, A. C. Eletroforese e marcadores bioquímicos em plantas e microorganismos. Viçosa, UFV, 2006.
BIEMELT, S.; KEETMAN, U.; ALBRECHT, G. Reaeration following hypoxia or anoxia leads to activation of the antioxidative defense system in roots of wheat seedlings. Plant Physiology, 116(2):651-658, 1998.
BONIECKA, J. et al. Potential biochemical, genetic and molecular markers of deterioration advancement in seeds of oilseed rape (Brassica napus L.). Industrial Crops and Products, 130:478-490, 2019.
BRADFORD, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2):248-254, 1976.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasilia: Ministério da Agricultura Pecuária e Abastecimento, 2009. 399p.
BUEGE, J. A.; AUST, S. D. Microsomal lipid peroxidation. Methods in Enzymology, 52(C):302-310, 1978.
CARVALHO, C. A. M.; ALMEIDA, T. T.; GUIMARÃES, R. M. Plântulas de café originadas de sementes armazenadas e submetidas ao condicionamento fisiológico em matriz sólida. Nativa, 2(3):166-169, 2014.
CATTANEO, C. et al. Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment. Scientific Reports, 11(1):3927, 2021.
CAVASIN, P. Y. et al. Endo-β-mannanase and superoxide dismutase as enzymatic markers for lettuce seeds thermotolerance. Journal of Seed Science, 45:e202345008, 2023.
CAVASIN, P. Y. et al. Endo-β-Mannanase enzyme activity is related to thermo inhibition in lactuca sativa seeds. Research Journal of Seed Science, 14(1):11-18, 2021.
COELHO, S. V. B. et al. Alterações fisiológicas e bioquímicas em sementes de café secas em sílica gel e soluções salinas saturadas. Pesquisa Agropecuaria Brasileira, 50(6):483-491, 2015.
DHINDSA, R. S.; PLUMB-DHINDSA, P.; THORPE, T. A. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32(1):93-101, 1981.
ELLIS, R. H.; HONG, T. D.; ROBERTS, E. H. An intermediate category of seed storage behaviour? II. Effects of provenance, immaturity, and imbibition on desiccation-tolerance in coffee. Journal of Experimental Botany, 42(5):653-657, 1991.
ELSAYED, A. I. et al.The impact of drought stress on antioxidant responses and accumulation of flavonolignans in milk thistle (Silybum marianum (L.) Gaertn). Plants, 8(12):611, 2019.
ESPINDOLA, L. S. et al. Cellular and metabolic damage induced by desiccation in recalcitrant Araucaria angustifolia embryos. Seed Science Research, 4(2):193-201, 1994.
FANG, W.-C.; KAO, C. H. Enhanced peroxidase activity in rice leaves in response to excess iron, copper and zinc. Plant Science, 158(1-2):71-76, 2000.
FERREIRA, V. F. et al. Endo-β-mannanase enzyme activity in the structures of Coffeaarabica L. seeds under different types of processing and drying. Ciência Rural, 48(12):e20170839, 2018.
FILHO, J. M. Teste de envelhecimento acelerado. In: VIEIRA, R. D.; CARVALBHO, N. M. Vigor de sementes: Conceitos e testes. Londrina: ABRATES - Associação Brasileira de Tecnologia de Sementes, p.133-149, 1999.
FOX J.; WEISBERG, S. An R companion to applied regression. Third edition. Sage, Thousand Oaks CA. 2019. 481p.
FRANÇA-NETO, J. B.; KRZYZANOWSAKI, F. C.; HENNING, A. A. A importancia do uso de sementes de soja de alta qualidade. Informativo ABRATES, 20(1-2):37-38, 2010.
GIANNOPOLITS, C. N.; RIES, S. K. Superoxide dismutases: II. purification and quantitative relationship with water-soluble protein in seedlings. Plant Physiology, 59(2):315-318, 1977.
GOMES, L. J. C. et al. Secagem estacionária e intermitente e sua influência na qualidade das sementes de soja. Revista Scientia Rural, 27(1):1-10, 2023.
GUARALDO, M. M. S. et al. Priming with sodium nitroprusside and hydrogen peroxide increases cotton seed tolerance to salinity and water deficit during seed germination and seedling development. Environmental and Experimental Botany, 209:105294, 2023.
HAVIR, E. A.; MCHALE, N. A. Purification and characterization of an isozyme of catalase with enhanced-peroxidatic activity from leaves of Nicotiana sylvestris. Archives of Biochemistry and Biophysics, 283(2):491-495, 1990.
HONG, T. D.; ELLIS, R. H. A comparison of maturation drying, germination, and desiccation tolerance between developing seeds of Acer pseudoplatanus L. and Acer platanoides L. New Phytologist, 116(4):589-596, 1990.
KASSAMBARA, A. rstatix: Pipe-friendly framework for basic statistical tests. R package version 0.7.2, 2023. Available in: <https://CRAN.R-project.org/package=rstatix>. Access in: 27 August 2024.
KUREK, K.; PLITTA-MICHALAK, B.; RATAJCZAK, E. Reactive oxygen species as potential drivers of the seed aging process. Plants, 8(6):174, 2019.
LIU, B.; SOUNDARARAJAN, P.; MANIVANNAN, A. Mechanisms of silicon-mediated amelioration of salt stress in plants. Plants, 8(9):1-13, 2019.
LIU, J. et al. High temperature and drought stress cause abscisic acid and reactive oxygen species accumulation and suppress seed germination growth in rice. Protoplasma, 256(5):1217-1227, 2019.
MAGUIRE, J. D. Speed of germination- Aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2):176-177, 1962.
MORENO, K. A. A. et al. Gene expression related to physiological quality of soybean seeds. Journal of Agricultural Science, 11(3):370, 2019.
NAKANO, Y.; ASADA, K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5):867-880, 1981.
NISHIZAWA, K.; KOMATSU, S. Characteristics of soybean 1-Cys peroxiredoxin and its behavior in seedlings under flooding stress. Plant Biotechnology, 28(1):83-88, 2011.
OLIVEIRA, T. F. et al. Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination. Journal of Seed Science, 44:e202244044, 2022.
OLIVER, M. J. et al. Desiccation tolerance: avoiding cellular damage during drying and rehydration. Annual Review of Plant Biology, 71:435-460, 2020.
PARKHEY, S.; NAITHANI, S. C.; KESHAVKANT, S. ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging. Plant Physiology and Biochemistry, 57:261-267, 2012.
PETERSON, B. G.; CARL, P. Performance analytics: Econometric tools for performance and risk analysis. R package version 2.0.4, 2020. Available in: <https://CRAN.R-project.org/package=PerformanceAnalytics>. Access in: 27 August 2024.
R CORE TEAM. R: A language and environment for statistical computing. R: Foundation for Statistical Computing, Vienna, Austria. 2023. Available in: https://www.R-project.org/. Access in: 27 August 2024.
RATAJCZAK, E.; DIETZ, K. J.; KALEMBA, E. M. The occurrence of peroxiredoxins and changes in redox state in acer platanoides and acer pseudoplatanus during seed development. Journal of Plant Growth Regulation, 38(1):298-314, 2019.
REVELLE, W. psych: Procedures for psychological, psychometric, and personality research. Northwestern University, Evanston, Illinois. R package version 2.4.3, 2023. Available in: <https://CRAN.R-project.org/package=psych>. Access in: 27 August 2024.
ROSA, S. D. V. F. et al. Enzimas removedoras de radicais livres e proteínas lea associadas à tolerância de sementes milho à alta temperatura de secagem. Revista Brasileira de Sementes, 27(2):91-101, 2005.
SADAK, M. S. Nitric oxide and hydrogen peroxide as signalling molecules for better growth and yield of wheat plant exposed to water deficiency. Egyptian Journal of Chemistry, 65(11):209-223, 2022.
SADAK, M. S.; BAKHOUM, G. S. Selenium-induced modulations in growth, productivity and physiochemical responses to water deficiency in Quinoa (Chenopodium quinoa) grown in sandy soil. Biocatalysis and Agricultural Biotechnology, 44:102449, 2022.
SCHNEIDER, C. A.; RASBAND, W. S.; ELICEIRI, K. W. NIH image to imageJ: 25 years of image analysis. Nature Methods, 9(7):671-675, 2012.
SILVA, B. M. C. et al. Influência da secagem na qualidade fisiológica e coloração das sementes de feijão guandu. Research, Society and Development, 9(7):e975974789, 2020.
SOUZA, D. N. et al. Wheat yield and seed physiological quality as influenced by seed vigor, nitrogen fertilization and edaphoclimatic conditions. Semina:Ciencias Agrarias, 42(6):3581-3602, 2021.
TAVEIRA, J. H. S. et al.Perfis proteicos e desempenho fisiológico de sementes de café submetidas a diferentes métodos de processamento e secagem. Pesquisa Agropecuária Brasileira, 47(10):511-1517, 2012.
TESFA, M. Review on postharvest processing operations affecting coffee (Coffea Arabica L.) quality in Ethiopia. Journal of Environment and Earth Science, 9(12):30-39, 2019.
VARNAVIDES, G. et al. In search of a universal method: a comparative survey of bottom-up proteomics sample preparation methods. Journal of Proteome Research, 21(10):2397-2411, 2022.
VITIS, M. et al. Seed storage: Maintaining seed viability and vigor for restoration use. Restoration Ecology, 28(S3):S249-S255, 2020.
WEI, S. et al. Comparative transcriptomics and metabolomics analyses provide insights into thermal resistance in lettuce (Lactuca sativa L.). Scientia Horticulturae, 289:110423, 2021.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Coffee Science - ISSN 1984-3909Os direitos autorais dos artigos publicados nesta revista pertencem aos autores, com os primeiros direitos de publicação pertencentes à revista. Como os artigos aparecem nesta revista com acesso aberto, eles podem ser usados livremente, com as devidas atribuições, em aplicativos educacionais e não comerciais.
