Aroma profiles and cupping characteristics of coffee beans processed by semi carbonic maceration process


  • Wanphen Jitjaroen Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang, Thailand.
  • Rungtiwa Kongngoen Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang, Thailand.
  • Lachinee Panjai Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Lampang, Thailand.



The goal is to improve the flavors of Arabica coffee by combining semi-carbonic maceration (SCM) process, with enzymatic process at various lengths and temperature. Five different variations of SCM processes were investigated: pulped natural process (control); at 15 oC for 10 days (SCM-15); at 20 oC for 10 days (SCM-20); with 0.01 g.L-1 pectinases A (polygalacturonase, pectin esterase and pectin lyase) at 20 oC for 5 days (SCM-PA); and with 0.03 g.L-1 pectinases B (polygalacturonase, pectin esterase, pectin lyase and ß-glucanase) at 20 oC for 5 days (SCM-PB). The chemical composition of intact mucilage and green bean coffee were analyzed. The environments created by using SCM fermentation process at 20 oC combined with pectinase A and pectinase B produced green bean coffee with higher content of acidity and reducing sugar. The volatile compounds of roasted coffee were examined by Gas Chromatography-Mass Spectrometry (GC-MS). Fifty-one volatile compounds belonging to 12 chemical classes were identified. The different total peak area, indicates that most volatile compounds, increases during SCM, and SCM process combined with enzymatic method. The Specialty Coffee Association of America; SCAA sensory analysis was performed by trained specialty coffee judges. The final product of coffee beans, which has been treated with SCM process at 15 and 20 oC have obtained the highest score in fragrance, flavor, aftertaste, body, balance and overall impression. While the enzyme treatment has attained the highest scores in acidity. This study demonstrates that the proposed process may be useful for the improvement of the cup quality.

Key words: Coffee; semi-carbonic maceration process; pectinase; gas chromatography-mass spectrometry; cup quality.


AGRESTI, P. D. M. et al. Discrimination between defective and non-defective Brazilian coffee beans by their volatile profile. Food Chemistry, 106(2):787-796, 2008.

AKIYAMA, M. et al. Analysis of the headspace volatiles of freshly brewed Arabica coffee using solid-phase microextraction. Journal of Food Science, 72(7):388-396, 2007.

AKIYAMA, M. et al. Characterization of flavor compounds released during grinding of roasted robusta coffee beans. Food Science and Technology Research, 11(3):298-307, 2005.

AMORIM, H. V.; AMORIM, V. L. Coffee enzyme and coffee quality. In: ORIJ, R. L.; ST ANGELO A. J. (Eds.). Enzymes in food and beverage processing. Indiana: American Chemical Society, p. 27-56, 1977.

ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. V.69. No. 2. 17th ed. Gaithersburg, Md, 2000. 233p.

ARYA, M.; RAO, L. J. An impression of coffee carbohydrates. Critical Reviews in Food Science and Nutrition, 51(1):51-67, 2007.

BAGGENSTOSS, J. et al. Roasting and aroma formation: Effect of initial moisture content and steam treatment. Journal of Agricultural and Food Chemistry, 56:5847-5851, 2008.

BASSOLI, D. R.; SILVA, S. F. Key aroma compounds of soluble coffee. 2019. 348p.

BELITZ, H. D.; GROSCH, W.; SCHIEBERLE, P. Food Chemistry. Berlin, Springer, p.938-970, 2009. Available in:

. Access in: August, 22, 2021.

BRESSANELLO, D. et al. Coffee aroma: Chemometric comparison of the chemical information provided by three different samplings combined with GC-MS to describe the sensory properties in cup. Food Chemistry, 214:218-226, 2017.

BRIOSCHI, J. D. et al. Microbial fermentation affects sensorial, chemical, and microbial profile of coffee under carbonic maceration. Food Chemistry, 342:128296, 2021.

CAPORASOA, N. et al. Variability of single bean coffee volatile compounds of arabica and robusta roasted coffees analysed by SPME-GC-MS. Food Research International, 108:628-640, 2018

CECCHINI, F. et al. The potential use of yeast lees (1-3, 1-6)-B glucan as functional food ingredients. Internet journal of Enology and Viticulture. 1-5, 2016. Available in: <>. Access in: August, 22, 2021.

COFFEE RESEARCH INSTITUTE. Coffee chemistry: Coffee aroma. 2001. Available in: <>. Access in: August, 2, 2021.

COSTE, R. Coffee: The plant and the product. London, MacMillan Press Ltd., 1992. 328p.

CUSIELO, K. V. C. et al. Sensory influence of sweetener addition on traditional and decaffeinated espresso. Journal of Food Science, 84(9):2628-2637, 2019.

CZERNY, M.; GROSCH, W. Potent odorants of raw arabica coffee. Their changes during roasting. Journal of Agricultural and Food Chemistry, 48(3):868-872, 2000.

CZERNY, M.; MAYER, F.; GROSCH, W. Sensory study on the character impact odorants of roasted arabica coffee. Journal of Agricultural and Food Chemistry, 47(2):695-699, 1999.

DINNELLA, C.; MASI, T.; NAES, E. M. A new approach in TDS data analysis: A case study on sweetened coffee. Journal of Food Science, 30(1):33-46, 2013.

DORFNER, al. Real-time monitoring of 4-vinylguaiacol, guaiacol, and phenol during coffee roasting by resonant laser ionization time-of-flight mass spectrometry. Journal of Agricultural and Food Chemistry, 51(19):5768-5773, 2003.

DUBOURDIEU, D. et al. Dégradationenzymatique du glucane de Botrytis cinerea. Application à l’amélioration de la clarification des vinsissus de raisins pourris. Connaissance de la Vigne et Du Vin, 15(3):161-177, 1981.

FRANCA, A. S. A Preliminary evaluation of the effect of processing temperature on coffee roasting degree assessment. Journal of Food Engineering, 92(3):345-352, 2009.

GROSCH, W. Evaluation of the key odorants of foods by dilution experiments, aroma models and omission. Chemical Senses, 26(5):533-545, 2001.

GUDI, P. Carbonic maceration (A unique way of coffee processing). Maceracion, 1-4, 2017.

GUOWAN, S.; ZHENG, L.; ZHAO, M. Characterization of antioxidant activity and volatile compounds of Maillard reaction products derived from different peptide fractions of peanut hydrolysate. Food Research International, 44(10):3250-3258, 2011.

GUNES, G.; BLUM, L. K.; HOTCHKISS, J. H. Inactivation of yeasts in grape juice using a continuous dense phase carbon dioxide processing system. Journal of the Science of Food and Agriculture, 85(14):2362-2368, 2005.

HAILE, M.; KANG, W. H. Isolation, identification, and characterization of pectinolytic yeasts for starter culture in coffee fermentation. Microorganisms, 7(10):401, 2019.

HERTZ-SCHÜNEMANN, R. et al. On-line process monitoring of coffee roasting by resonant laser ionisation time-of-flight mass spectrometry: Bridging the gap from industrial batch roasting to flavour formation inside an individual coffee bean. Journal of Mass Spectrometry, 48(12):1253-1265, 2013.

HUMBERT-GOFFARD, A. An assay for glucanase activity in wine. Enzyme and Microbial Technology, 34:537-543, 2004.

HÜFNER, E.; HAßELBECK, G. Application of microbial enzymes during winemaking. In: KÖNIG, H.; UNDEN, G.; FRÖHLICH, J. Eds., Biology of microorganisms on grapes, in must and in wine. Cham: Springer International Publishing, p. 635-658, 2017.

JITJAROEN, W.; CHAISRI, D.; PANJAI, L. Characterization of active-aroma wheel in contemporary coffee processes via gas chromatography: Olfactometry, and sensory perspective. Coffee Science, 18:e182059, 2023.

KAMIYAMA, M. et al. Role of degradation products of chlorogenic acid in the antioxidant activity of roasted coffee. Journal of Agricultural and Food Chemistry, 63(7):1996-2005, 2015.

KASHYAP, D. R. et al. Applications of pectinases in the commercial sector: A review. Bioresource Technology, 77(3):215-227, 2001.

KUBRA, K. T. et al. Potential applications of pectinases in food, agricultural and environmental sectors. Journal of Pharmaceutical, Chemical and Biological Sciences, 6(2):23-34, 2018.

KUMAZAWA, K. Flavor chemistry of tea and coffee drinks. Food Science and Technology Research, 12(2):71-84, 2006.

LAI, Y-T. et al. Isolation and identification of aroma producing strain with esterification capacity from yellow water. PLOS ON, 14(2):e0211356, 2019.

LINGLE, T. R. The coffee cupper’s handbook: Systematic guide to the sensory evaluation of coffee’s flavor, 4th ed. Specialty Coffee Association of America. Long Beach: CA, 2011. 66p.

LIU, L. Et al. Effect of carbonic maceration pre-treatment on drying kinetics of chilli (Capsicum annuum L.) flesh and quality of dried product. Food Bioprocess Technology, 7(9):2516-2527, 2014.

MAEZTU, L. et al. Characterization of espresso coffee aroma by static headspace GC-MS and sensory flavor profile. Journal of Agricultural and Food Chemistry, 49(11):5437-5444, 2001.

MAKRI, E. et al. Modeling of Greek coffee aroma loss during storage at different temperatures and water activities. Procedia Food Science, 1:1111-1117, 2011.

MANZOCCO, L.; LAGAZIO, C. Coffee brew shelf life modelling by integration of acceptability and quality data. Food Quality and Preference, 20(1):24-29, 2009.

MEENAKSHI, A.; JAGAN, L. An impression of coffee carbohydrates. Food Science and Nutrition, 47(1):51-67, 2007.

MIN, J. S.; KWON, H. M.; PARK, S. K. Impacts of coffee creamer, dried skim milk and sugar on the volatile aroma compounds and sensory characteristics in instant coffee. The Korean Journal of Food Science and Technology, 47(2):137-144, 2015.

MOJSOV, K. Use of enzymes in wine making: A review. International Journal of Technology Marketing, 3(9):112-127, 2013.

MOJSOV, K. Enzymes and wine-The enhanced quality and yield. Advances in Technology, 4:94-100, 2015.

MOON, J. K.; SHIBAMOTO, T. Role of roasting conditions in the profile of volatile flavor chemicals formed from coffee beans. Journal of Agricultural and Food Chemistry, 57(13):5823-5831, 2009.

PALOMERO, F. et al. Conventional and enzyme-assisted autolysis during ageing over lees in red wines: Influence on the release of polysaccharides from yeast cell walls and on wine monomeric anthocyanin content. Food Chemistry, 105:838-846, 2007.

PEREIRA, P. V. et al. Microbial diversity and chemical characteristics of Cofea canephora grown in different environments and processed by dry method. World Journal of Microbiology and Biotechnology, 37(51):1-12, 2021.

PIMENTA, T. V. et al. Roasting processing of dry coffee cherry: Influence of grain shape and temperature on physical chemical and sensorial grain properties. Boletim do Centro de Pesquisa de Processamento de Alimentos, 27(1):97-106, 2009.

POLTRONIERI, P.; ROSSI, F. Challenges in specialty coffee processing and quality assurance. Challenges, 7:01-19, 2016.

REBEIRO, L. S. et al. Controlled fermentation of semi-dry coffee (Coffea arabica) using starter cultures: A sensory perspective. LWT - Food Science and Technology, 82:32-38, 2017.

RETA. et al. Reducing the acidity of Arabica coffee beans by ohmic fermentation technology. Food Research, 1(5):157-160, 2017.

RITTHIRUANGDEJ, P. Data analysis for research and product development using SPSS. Vista Inter Print (in Thai), 2018.375 p.

RIZZI, G. P.; SANDERS, R. A. Mechanism of pyridine formation from trigonelline under coffee roasting conditions. Royal Society of Chemistry, 197:206-210, 1996.

ROBINSON, J. The oxford companion to wine. 3rd. Ed. Oxford University Press. 2006. 780p.

RODRIGUEZ-NOGALES, J. M.; FERNÁNDEZ-FERNÁNDEZ, E.; VILA-CRESPO, J. Effect of the addition of ß-glucanases and commercial yeast preparations on the chemical and sensorial characteristics of traditional sparkling wine. European Food Research and Technology, 235: 729-744, 2012.

SCHENKER, S. et al. Impact of roasting conditions on the formation of aroma compounds in coffee beans. Journal of Food Science, 67(1):60-66, 2002.

SERRAT, M.; BERMÚDEZ, R. C.; VILLA, T. G. Production, purification and characterization of a polygalacturonase from a new strain of Kluyveromyces marxianus isolated from coffee wet-processing wastewater. Applied Biochemistry and Biotechnology, 97:193-208, 2002.

SILV, C. F. et al. Microbial diversity during maturation and natural processing of coffee cherries of Coffea arabica in Brazil. International Journal of Food Microbiology, 60(2-3):251-260, 2000.

SUNARHARUM, W. B.; WILLIAMS, D. J.; SMYTH, H. E. Complexity of coffee flavor: A compositional and sensory perspective. Food Research International, 62:315-325, 2014.

TESNIERE, C.; FLANZY, C. Carbonic maceration wines: Characteristics and winemaking process. Advances in Food and Nutrition Research, 63:01-15, 2011.

THAMMARAT, P. et al. Identification of volatile compounds and selection of discriminant markers for elephant dung coffee using static headspace Gas chromatography-mass spectrometry and chemometrics. Molecules, 23(8):01-14, 2018.

THE GOOD SENSE COMPANY. The good scents company information system

providing information for the flavor, fragrance, food and cosmetic industries. 2019. Available in:

<>Access in: August, 22, 2021.

SPECIALTY COFFEE ASSOCIATION - SCA. Protocol & best practice. 2021. Available in: <>. Access in: August, 2, 2021.

VAN BOEKEL, M. A. J. S. Formation of flavor compounds in the Maillard reaction. Biotechnology Advances, 24:230-233, 2006.

VARELAS, V. et al. Production of OF β-Glucan from winery yeast waste biomass. University of Athens Department of Chemistry. 1-16, 2016. Available in:

<>. Access in: August,15, 2021.

WESCHENFELDER, T. A. et al. Concentration of aroma compounds from an industrial solution of soluble coffee by pervaporation process. Journal of Food Engineering, 159:57-65, 2015.

WHITAKER, J. R. Microbial pectinolytic enzymes. In: FOGARTY, W. M.; KELLY, C. T. Eds., Microbial enzymes and biotechnology. London: Elsevier Science Ltd. p.133-176, 1990.

YANG, N. et al. Determination of volatile marker compounds of common coffee roast defects. Food Chemistry, 211:206-214, 2016.

YERETZIAN, C. et al. From the green bean to the cup of coffee: Investigating coffee roasting by on-line monitoring of volatiles. European Food Research and Technology, 214(2):92-104, 2002.




How to Cite

JITJAROEN, W.; KONGNGOEN, R.; PANJAI, L. Aroma profiles and cupping characteristics of coffee beans processed by semi carbonic maceration process. Coffee Science - ISSN 1984-3909, [S. l.], v. 18, p. e182119, 2023. DOI: 10.25186/.v18i.2119. Disponível em: Acesso em: 30 sep. 2023.