In vivo compatibility between Beauveria bassiana (Bals.) Vuillemin and castor oil on Hypothenemus hampei (Ferrari)




The degree of in vivo compatibility between the entomopathogenic fungi Beauveria bassiana (Bals.) Vuillemin (Ascomycota: Hypocreales) and castor oil is important, as the interaction can be additive, synergistic or antagonistic. Thus, the objective of this study was to evaluate the in vivo compatibility between castor oil and B. bassiana on the coffee berry borer. The CCA-UFES/Bb-4 isolate of B. bassiana was used in concentrations of 0.0 (control), 1 x 104, 1 x 105,
1 x 106 and 1 x 107 conidia mL-1. The concentrations of castor oil were: 0.0 (control), 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% v v-1. The total and confirmed mortality were evaluated, and data submitted to variance analysis. To verify the effect of B. bassiana and castor oil concentrations, the data were submitted to regression analysis at 5% probability. Castor oil had an antagonistic effect on B. bassiana reducing the mortality of coffee berry borer. The mortality of H. hampei reduced caused by interaction between B. bassiana and castor oil, due to increasing concentration of castor oil. Lower interference of castor oil was observed for high concentrations of B. bassiana. Based on the results, management of H. hampei cannot be normally performed using the association between castor bean oil and B. bassiana, except if the economic viability of the higher concentrations justified.

Key words: Ricinus communis; Botanical insecticides; Biological control; Phytosanitary pest management; Coffee crop.

Author Biography

Flávio Neves Celestino, Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de Minas Gerais/IFSUDESTE-MG, Campus Manhuaçu, Manhuaçu, MG.

Formação acadêmica/titulação: Engenheiro Agrônomo, Doutor em Produção Vegetal Instituição/Afiliação: Engenheiro Agrônomo do Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, Instituto Federal do Espírito Santo - Campus Montanha. 


ALVES, R.T.; FARIA, M. Pequeno manual sobre fungos entomopatogênicos. Documento 286, Planaltina: Embrapa Cerrados, 2010. 50p.

ARAUJO JR, J. M. de.; MARQUES, E. J.; OLIVEIRA, J. V. de. Potencial de isolados de Metarhizium anisopliae e Beauveria bassiana e do Óleo de Nim no Controle do Pulgão Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae). Neotropical Entomology, 38(4):520-525, 2009.

ARNOSTI, A. et al. Effects of ricinoleic acid esters from castor oil of Ricinus communis on the vitellogenesis of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks. Experimental Parasitology, 127(2):575-580, 2011.

BIGI, M. F. M. A. et al. Activity of Ricinus communis (Euphorbiaceae) and ricinine against the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera: Formicidae) and the symbiotic fungus Leucoagaricus gongylophorus. Pest Management Science, 60(9):933-938, 2004.

CAMPOS, E. V. R. et al. Use of botanical insecticides for sustainable agriculture: Future perspectives. Ecological Indicators, 105:483-495, 2019.

CELESTINO, F. N. et al. Toxicidade do óleo de mamona à broca-do-café [Hypothenemus hampei (FERRARI) (COLEOPTERA: CURCULIONIDAE: SCOLYTINAE)] [Toxicity of castor oil to coffee berry borer [Hypothenemus hampei (FERRARI) (COLEOPTERA: CURCULIONIDAE: SCOLYTINAE)]]. Coffee Science, 10(3):329 - 336, 2015.

CELESTINO, F. N. et al. Control of coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) with botanical insecticides and mineral oils. Acta Scientiarum. Agronomy, 38(1):1-8, 2016a.

CELESTINO, F. N. et al. Adaptação de técnicas de criação da broca-do-café [Hypothenemus hampei (Ferrari)] [Adaptation of technique for rearing of the coffee berry borer [Hypothenemus hampei (Ferrari)]]. Coffee Science, 11(2):161-168, 2016b.

CELESTINO, F. N. et al. Compatibilidade in vitro entre Beauveria bassiana e o óleo de mamona. Revista Brasileira de Ciências Agrárias, 13(4):e5595, 2018.

CORREAL, C. E. et al. Hongos entomopatógenos en el control biológico de insectos plaga. In: COTES, A. M. Control biológico de fitopatógenos, insectos y ácaros. Mosquera, (Colombia): Agrosavia, 2018. 568p.

DALVI, L. P. et al. Selection of native isolates of Beauveria bassiana (Ascomycota, Hypocreales) for the control of the coffee borer beetle Hypothenemus hampei (Scolytinae) in Brazil. Biological Letters, 48(1):39-46, 2011.

DARA, S. K. The New Integrated Pest Management Paradigm for the Modern Age. Journal of Integrated Pest Management, 10(1):1-9, 2019.

DEPIERI, R. A.; MARTINEZ, S. S.; MENEZES JR. A. O. Compatibility of the Fungus Beauveria Bassiana (Bals.) Vuill. (Deuteromycetes) with Extracts of Neem Seeds and Leaves and the Emulsible Oil. Neotropical Entomology, 34(4):601-606, 2005.

FANG, W. et al. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulent. Applied and Environmental Microbiology, 71(1):363-370, 2005.

ISLAM, M. T.; CASTLE, S. J.; REN, S. Compatibility of the insect pathogenic fungus Beauveria bassiana with neem against sweetpotato whitefly, Bemisia tabaci, on eggplant. Entomologia Experimentalis et Applicata, 134(1):28-34, 2010.

ISLAM, M. T.; OLLEKA, A.; REN, S. Influence of neem on susceptibility of Beauveria bassiana and investigation of their combined efficacy against sweetpotato whitefly, Bemisia tabaci on eggplant. Pesticide Biochemistry and Physiology, 98(1):45-49, 2010.

ISLAM, M.T.; OMAR, D. B. Combined effect of Beauveria bassiana with neem on virulence of insect in case of two application approaches. The Journal of Animal & Plant Sciences, 22(1):77-82, 2012.

ISMAN, M. B.; GRIENEISEN, M. L. Botanical insecticide research: many publications, limited useful data. Trends in Plant Science - Cell Press, 19(3):140-145, 2014.

MACHADO, L. C. et al. Toxicidade de óleos minerais e vegetais no manejo de Planococcus citri [Toxicity of mineral and vegetable oils in management of Planococcus citri]. Acta Ambiental Catarinense, 17(1):63-72, 2020.

MINISTÉRIO DA AGRICULTURA, PECUÁRIA E ABASTECIMENTO - MAPA. Valor Bruto da Produção Agropecuária de 2019 é atualizado para R$ 609,5 bilhões. 2019. Available in: <>. Access in: May,18, 2020a.

MINISTÉRIO DA AGRICULTURA, PECUÁRIA E ABASTECIMENTO - MAPA. AGROFIT: Sistema de agrotóxicos fitossanitários. 2020. Available in: <>. Access in: May,18, 2020b.

MOHAN, M. C. et al. Growth and insect assays of Beauveria bassiana with neem to test their compatibility and synergism. Biocontrol Science and Technology, 17(10):1059-1069, 2007.

MOLINA, D. et al. Effects of the aspartic protease inhibitor from Lupinus bogotensis seeds on the growth and development of Hypothenemus hampei: An inhibitor showing high homology with storage proteins. Phytochemistry, 98:69-77, 2014.

NASCIMENTO, V. V. DO et al. In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds. Journal of Agricultural and Food Chemistry, 59(9):4814-4821, 2011.

INTERNATIONAL COFFEE ORGANIZATION - OIC. Growing Prosperity - Economic viability as the catalyst for a sustainable coffee sector. 2019. London: International Coffee Organization - Coffee Development Report, Available at: <>. Accessed on: May, 18, 2020.

OLIVEIRA, C. M. et al. Economic impact of exotic insect pests in Brazilian agriculture. Journal of Applied Entomology, 137(1-2): 1-15, 2013.

OVERMEER, W. P. J.; VAN ZON, A. Q. A standardized method for testing the side effect of pesticides on the predaceous mite, Amblyseius potentillae (Acari: Phytoseiidae). Entomophaga, 27(4):357-364, 1982.

PEDRINI, N. et al. Molecular characterization and expression analysis of a suite of cytochrome P450 enzymes implicated in insect hydrocarbon degradation in the entomopathogenic fungus Beauveria bassiana. Microbiology, 156(8):2546-2557, 2010.

RAMOS-LÓPEZ, M.A. et al. Activity of Ricinus communis (Euphorbiaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae). African Journal of Biotechnology, 9(9): 1359-1365, 2010.

RONDELLI, V. M. et al. Associação do óleo de mamona com Beauveria bassiana no controle da traça-das-crucíferas. Pesquisa Agropecuária Brasileira, 46(2):212-214, 2011.

SAMPER, L.; GIOVANNUCCI, D.; MARQUES VIEIRA, L. The powerful role of intangibles in the coffee value chain. Geneva: World Intellectual Property Organization - Economics and Statistics Division, 2017. Available in: <>. Access in: May, 18, 2020.

SWATHI, P.; VISALAKSHY, P. N. G.; DAS, S. B. In vitro evaluation for compatibility of additives with Beauveria bassiana (Balsamo) Vuillemin. Egyptian Journal of Biological Pest Control, 28(13):1-5, 2018.

TOUNOU, A.K. et al. Bio-insecticidal effects of plant extracts and oil emulsions of Ricinus communis L. (Malpighiales: Euphorbiaceae) on the diamondback, Plutella xylostella L. (Lepidoptera: Plutellidae) under laboratory and semi-field conditions. Journal of Applied Biosciences, 43(3):2899-2914, 2011.

VEGA, F. E. et al. The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera:Curculionidae): a short review, with recent findings and future research directions. Terrestrial Arthropod Reviews, 2(2):129-147, 2009.

VEGA, F.E. et al. On the Eyes of male coffee berry borers as rudimentary organs. PLoS ONE, San Francisco, 9(1):858-860, 2014.

ZHANG, S. et al. CYP52X1, representing new cytochrome P450 subfamily, display fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus Beauveria bassiana. Journal of Biological Chemistry, 287(16):13477-13486, 2012.



How to Cite

CELESTINO, F. N.; PRATISSOLI, D.; GONÇALVES DOS SANTOS JUNIOR, H. J. .; CONTARINI MACHADO, L. In vivo compatibility between Beauveria bassiana (Bals.) Vuillemin and castor oil on Hypothenemus hampei (Ferrari). Coffee Science - ISSN 1984-3909, v. 15, p. e151771, 30 Dec. 2020.