Activity of robusta coffee on the expression of CRP and MMP-9 against hyperlipidemia




Hyperlipidemia especially LDL accumulation causing inflammation in blood vessel. CRP and MMP-9 are component that triggering in inflammation process. This study to know activity of robusta coffee against hyperlipidemia on this component. This study using male wistar rats were divided into three groups ; normal, hyperlipidemia and that were given coffee with high fat diet groups. Robusta coffee was given 3.6x10-6 m3 by intragastric gavage. Blood
collection was carried out using infra-orbital for analysis of LDL levels. Furthermore, the experimental animals were sacrificed and coronary arteries were taken for CRP and MMP-9 immunohistochemical staining. Data were analyzed using ANOVA followed LSD test. Results show The highest expression score for CRP and MMP-9 within the hyperlipidemia group was 188.27 ± 1.8 and 277.56 ± 2.1. The decrease in the expression score of CRP and MMP-9 happened within the ones that were given coffee with 59.33 ± 1.5 and 116.7 ± 0.2 each. Results showed that were differences in the expression of CRP and
MMP-9 in all groups (p <0.05). In conclusion, activity robusta coffee can decreases the expression of CRP and MMP-9 against hyperlipidemia.

Key words: Coronary disease; hyperlipidemia; robusta coffee.

Author Biography

Rendra Chriestedy Prasetya, University of Jember, Biomedical Department, Jember - Indonesia.

Biomedical Department


ANTHONY, S. F. et al. Harrison’s Principles of Internal Medicine Volume II. 18th Ed. United States of America: McGraw-Hill. 2012. 134p.

BENTZON, J. F. et al. Mechanisms of plaque formation and rupture. Circulation Research, 114(12):1852-1866, 2014.

CALABRO, P.; GOLIA, E.; YEH, E. T. CRP and the risk of atherosclerotic events. Seminars in Immunopathology,

:79-94, 2009.

CARDOSO, I. L.; PAULOS, A. N. C Reactive Protein (CRP) and cardiovascular disease. International Archives of Cardiovascular Disease, 1(1):1-11, 2017.

CHUNG, S. H.; MAKAMBI, K. H.; SOLDIN O. P. Tobacco smoke exposure, C-reactive protein and steroid hormones

measured by Tandem Mass Spectrometry in healthy women. Journal of Steroids and Hormonal Science, 5(4):1-6, 2014.

ECKMAN, D. M. et al. Weekly doxorubicin increases coronary arteriolar walland adventitial thickness. Plos One, 8(2):1-6, 2013.

FANG, Y. C. et al. Bioactivities of crude caffeine: Antioxidant activity, cyclooxygenase-2 inhibition, and enhanced glucose uptake. Food Chemistry, 131(2):564-568, 2012.

FARAH, A. Coffee: Emerging Health Effects and Disease Prevention. Oxford: Wiley- Blackwell, 2012. 45p.

FONSECA, A. L.; LIMA, F.; COUTO, R. D. The action of metaloproteinase in atherosclerosis diseases. ABCS

Health Science, 39(3):186-193, 2014.

HARSA, I. M. S. Efek Pemberian Diet Tinggi Lemak terhadap Profil Lemak Darah Tikus Putih (Rattus Norvegicus).

Jurnal Ilmiah Kedokteran, 3(1):21-28, 2014.

INDRIANI, V.; SAPUTRA, H.; MAKER, I. I. P. L. Poly (ADP Ribose) polymerase-1 Overexpression as A Predictive Factor for Poor Chemotheraphy Outcome in Triple Negative Breast Cancer. International Journal of Science and Research, 6(11):1881-1885, 2017.

JIN, U. H. et al. A phenolic compound, 5- caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix

metalloproteinase-9 inhibitor: Isolation and identification from methanol extract of Euonymus alatus. Life Sciences,

(9):60-67, 2005.

KRAKAUER, T. The polyphenol chlorogenic acid inhibits staphylococcal exotoxin-induced inflammatory cytokines

and chemokines. Immunopharmacol Immunotoxicol, 24(9):113-120, 2002.

LAMPIASI, N.; MONTANA, G. The molecular events behind ferulic acid mediated modulation of IL-6 expression in LPS-activated Raw 264.7 cells. Immunobiology, 221(4):86-93, 2016.

LEE, K.; LEE, B. J.; BU, Y. Protective effect of dihydrocaffeic acid, a coffee component metabolite, on a focal cerebral

ischemia rat model. Molecules, 119(20):30-40, 2015.

MARCO, L. M.; FISCHER, S.; HENLE, T. High molecular weight coffee melanoidins are inhibitors for matrix metalloproteases. Journal of Agricultural and Food Chemistry, 12(59):417-423. 2011.

MONTERO, I. et al. C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells:

Implications for clinical and subclinical atherosclerosis. Journal of the American College of Cardiology,

(7):1369-1378, 2006.

NEWBY, A. C. Role of metalloproteinase in plaque rupture. International Journal of Gerontology, 1(3):103-111, 2015.

SHIN, H. S. et al. Catechol Groups Enable Reactive Oxygen Species Scavenging-Mediated Suppression of PKDNFkappaB-

IL-8 Signaling Pathway by Chlorogenic and Caffeic Acids in Human Intestinal Cells. Journal of

Nutrient, 9(2):66-72, 2017.

SILVA, E. O.; BATISTA, R. Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: A review

on their structures, occurrence, and potential health benefits. Comprehensive Reviews in Food Science and

Food Safety, 16(2):580-616, 2017.

SUDOYO, A. W. et al. Buku Ajar Ilmu Penyakit Dalam. Jakarta: Interna Publishing. 2010. p.1984-1992.

WU, M. Y. et al. New insight into the role of inflammation in the pathogenesis of atherosclerosis. International

Journal of Molecular Science, 18:1-18, 2017.

WULANDARI, L. R. et al. Efek pemberian matrix metalloproteinase-9 (MMP- 9) RNA interference terhadap

ekspresi MMP-9 pada kultur sel endotel vaskular. Jurnal Kedokteran Brawijaya, 22(6):50-57, 2016.

YABLUCHANSKIY, A. et al. Matrix metalloproteinase-9: Many shades of function in cardiovascular disease.

Physiology, 28(5):391-403, 2012.



How to Cite

PRASETYA, R. C.; FATIMATUZZAHRO, N. .; JATMIKO, W. . Activity of robusta coffee on the expression of CRP and MMP-9 against hyperlipidemia. Coffee Science - ISSN 1984-3909, v. 15, p. e151744, 3 Jul. 2020.