PHIALOMYCES MACROSPORUS REDUCES CERCOSPORA COFFEICOLA SURVIVAL ON SYMPTOMATIC COFFEE LEAVES

Marie Caroline Ferreira Laborde, Deila Magna dos Santos Botelho, Gabriel Alfonso Alvarez Rodriguez, Mário Lúcio Vilela de Resende, Marisa Vieira de Queiroz, Aline Duarte Batista, Patrícia Gomes Cardoso, Sérgio Florentino Pascholati, Luis Fernando Pascholati Gusmão, Samuel Júlio Martins, Flávio Henrique Vasconcelos de Medeiros

Abstract


Saprobe fungi and necrotrophic pathogens share the same niche within crop stubble and the search for fungi non-pathogenic to plants that are able to displace the plant pathogens from its overwintering substrate contributes to the disease management. Brown eye spot (Cercospora coffeicola) is among the most important coffee diseases, it is caused by a necrotrophic pathogen that has decaying leaves as its major source of inoculum. We have screened saprobe fungi for the ability to reduce C. coffeicola sporulation and viability and determined the possible mechanisms involved in the observed biocontrol. A selected saprobe fungus, Phialomyces macrosporus, reduced the pathogen’s viability by 40% both in vitro and in vivo. The fungus acts through antibiosis and competition for nutrients. It produced both volatile and non-volatile compounds that inhibited C. coffeicola growth, sporulation, and viability. It also produced the tissue maceration enzyme (polygalacturonase), which reduces the pathogen both in detached leaves or in planta. The reduction in the fungal viability either by the saprobe fungus or its polygalacturonase-fraction supernatant resulted in the reduction of the disease rate. Therefore, P. macrosporus is a potential microbial agent that can be used in an integrated management of brown eye spot through the reduction of the initial inoculum of the pathogen that survives and builds up in infected leaves.

 


Keywords


Coffea arabica, competição, controle biológico, macha de olho pardo, antibiose

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References


ABE, H. et al. Total synthesis of the proposed structure of a polyketide from Phialomyces macrosporus. Chemical Communication. v. 51, n. 17, p. 3586-3589. January. 2015.

AGROFIT. Sistema de agrotóxicos Fitossanitários. Available on :< http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons> Accessed November 26, 2017.

AHMAD, R.; THARAPPAN, B.; BONGIRWAR, D.R. Impact of gamma irradiation on the monsooning of coffee beans. Journal of Stored Products Research. v.39, n.2, p. 149-157. November. 2003.

BARAHONA, E. et al. Pseudomonas fluorescens F113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens. Applied and environment microbiology. v. 7, n. 77, p. 5412-5419. June. 2011.

BECKMAN, P. M.; PAYNE, G. A. Cultural techniques and conditions influencing growth and sporulation of Cercosporazeae-maydis and lesion development in corn. Phytopathology. v. 73, n. 2, p. 286-289. August. 1983.

BELLOTTE, J. A. M. et al. Acceleration of the decomposition of Sicilian lemon leaves as an auxiliary measure in the control of citrus black spot. Tropical Plant Pathology. v. 34, n. 2, p. 71-76. April. 2009.

BOTREL, D. A. Fungos sapróbios do semiárido nordestino como agentes de biocontrole da mancha aureolada. 2013. 57 p. Dissertação (Thesis in Agronomy/ Plant disease) - Universidade Federal de Lavras, Lavras, 2013.

CALVO‐GARRIDO, C. et al. Suppression of Botrytis cinerea on necrotic grapevine tissues by early‐season applications of natural products and biological control agents. Pest management science. v.70, n. 4, p. 595-602. July. 2014.

CARDOSO, R. M. D. L. et al. Efficiency of green manures for Cercospora leaf spot management in coffee plants. Tropical Plant Pathology. v.38, n. 2, p. 122-127. April. 2013.

DE CARVALHO. et al. Modeling spatial variability and pattern of rust and brown eye spot in coffee agroecosystem. Journal of Pest Science. v. 82, n. 1, p. 137. May. 2009.

CERDA, R. et al.Primary and secondary yield losses caused by pests and diseases: Assessment and modeling in coffee. PloS one. v.12, n. 1, p133-169. January. 2017.

FALMY, A. S. et al. Characterization of an exopoly galacturonase from Aspergillus niger. Applied Biochemestry and Biotechnology. v.149, n. 3, p. 205-217. June. 2008.

GALLETTI, S.et al. Trichoderma as a potential biocontrol agent for Cercospora leaf spot of sugar beet. BioControl. v. 53, n. 3, p. 917-930. September.2008.

GARG, G. et al. Microbial pectinases: an ecofriendly tool of nature for industries. 3 Biotech. v. 6, n. 47, p. 1–13. February. 2016.

HAUPTMAN, T. et al. Application of fungicides and urea for control of ash dieback. Journal of Biogeosciences and Forestry. v.8, n. 2, p. 1-7. August. 2015.

HIRADATE, S. et al. Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2. Phytochemistry. v.61, n. 6, p. 693-698. November. 2002.

KÖHL, J. et al. Effect of interrupted leaf wetness periods on suppression of sporulation of Botrytis-allii and Botrytis-cinerea by antagonists on dead onion leaves. European Journal of Plant Patholology. v.101, n. 6, p. 627-637. November.1995.

KÖHL, J. et al. Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria. Biol Control. v.57, n. 1, p. 1-12. April. 2011.

LIN, Y. et al. Priority colonization of Cinnamomum camphora litter by endophytes affects decomposition rate, fungal community and microbial activities under field conditions. Pedobiologia (Jena). v. 58, n. 6, p. 177-185. November. 2015.

MINUSSI, R. C. et al. Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum. Revista de microbiologia. v. 29, n. 4, p. 246-250. December.1998.

ODILE, C.; DANIEL, R.; DAVID-MATHIEU, T. Effect of Microsphaeropsisochracea on production of sclerotia-borne and airborne conidia of Botrytis squamosal. Biocontrol. v. 51, n. 1, p. 107-126. February.2006.

PRASAD, B. N., KUMAR, M.R. Effect of non-volatile compounds produced by Trichoderma spp. on growth and sclerotial viability of Rhizoctonia solani, incitant of sheath blight of rice. Indian Journal of Fundamental and Applied Life Science. v.1, n. 2, p. 37-42. June. 2011.

REZENDE, E. F. et al Potencial enzimático e toxigênico de fungos isolados de grãos de café. Coffee Science. v. 8, n. 1, p. 69-77. Mach. 2013.

RODRÍGUEZ, G. A. A. et al. Phialomyces macrosporus decreases anthracnose severity on coffee seedlings by competition for nutrients and induced resistance. Biological Control. v. 103, v.1. n. 119-128. December. 2016.

SIRINUNTA, A., AKARAPISAN, A. Screening of Antagonistic Bacteria for Controlling Cercospora coffeicola in Arabica Coffee. Journal of Agricultural Technology. v.11, n. 5, p. 1209-1218. July. 2015.

SOUZA, A. G. C., MIZUBUTII, E.S.G., MAFFIA, L.A. Esporulação ïn vitro de Cercospora coffeicola. Fitopatologia Brasileira. v.30, n 116. Jun.2005.

THRANE, C.; JENSEN, D.F.; TRONSMO, A.Substrate colonization, strain competition, enzyme production in vitro, and biocontrol of Pythium ultimum by Trichoderma spp. isolates P1 and T3. European Journal of Plant Pathology. v.106, n. 3, p. 215-225. March. 2000.

ZAMBOLIM, L.; VALE, F. X. R.; ZAMBOLIM, E. M. Doenças do cafeeiro (C.arabica e C. canephora). In: KIMATI, H.; AMORIM, L.; BERGAMIN FILHO, A (Ed.), Manual de fitopatologia: doenças das plantas cultivadas. São Paulo/Brazil, 2005, p 165-180.

ZHANG, X. Antibiosis functions during interactions of Trichoderma afroharzianum and Trichoderma gamsii with plant pathogenic Rhizoctonia and Pythium. Functional and Integrative genomics. v.15, n. 5, p. 599-610. September.2015.




DOI: http://dx.doi.org/10.25186/cs.v14i1.1448

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